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Angew Chem Int Ed Engl

Title:		Cryogenic OrbiSIMS Localizes Semi-Volatile Molecules in Biological Tissues
Author(s):		Newell CL; Vorng JL; MacRae JI; Gilmore IS; Gould AP;
Address:		"Physiology and Metabolism Laboratory, The Francis Crick Institute, 1 Midland Road, London, NW1 1AT, UK. NiCE-MSI, National Physical Laboratory, Hampton Road, Teddington, TW11 0LW, UK. Metabolomics Science Technology Platform, The Francis Crick Institute, 1 Midland Road, London, NW1 1AT, UK"
Journal Title:		Angew Chem Int Ed Engl
Year:		2020
Volume:		20200813
Issue:		41
Page Number:		18194 - 18200
DOI:		10.1002/anie.202006881
ISSN/ISBN:		1521-3773 (Electronic) 1433-7851 (Print) 1433-7851 (Linking)
Abstract:		"OrbiSIMS is a recently developed instrument for label-free imaging of chemicals with micron spatial resolution and high mass resolution. We report a cryogenic workflow for OrbiSIMS (Cryo-OrbiSIMS) that improves chemical detection of lipids and other biomolecules in tissues. Cryo-OrbiSIMS boosts ionization yield and decreases ion-beam induced fragmentation, greatly improving the detection of biomolecules such as triacylglycerides. It also increases chemical coverage to include molecules with intermediate or high vapor pressures, such as free fatty acids and semi-volatile organic compounds (SVOCs). We find that Cryo-OrbiSIMS reveals the hitherto unknown localization patterns of SVOCs with high spatial and chemical resolution in diverse plant, animal, and human tissues. We also show that Cryo-OrbiSIMS can be combined with genetic analysis to identify enzymes regulating SVOC metabolism. Cryo-OrbiSIMS is applicable to high resolution imaging of a wide variety of non-volatile and semi-volatile molecules across many areas of biomedicine"
Keywords:		"Cold Temperature History, 15th Century Mass Spectrometry/*methods Volatile Organic Compounds/*analysis analytical methods biological mass spectrometry imaging lipids semi-volatile molecules;"
Notes:		"MedlineNewell, Clare L Vorng, Jean-Luc MacRae, James I Gilmore, Ian S Gould, Alex P eng WT_/Wellcome Trust/United Kingdom 104566/Z/14/Z/WT_/Wellcome Trust/United Kingdom Historical Article Research Support, Non-U.S. Gov't Germany 2020/07/01 Angew Chem Int Ed Engl. 2020 Oct 5; 59(41):18194-18200. doi: 10.1002/anie.202006881. Epub 2020 Aug 13"