« Previous AbstractIon-dipole interaction for selective detection of acetone by perovskite BiFeO(3) chemi-resistive sensor    Next AbstractAnalytical methods for the screening of potential volatile migrants from acrylic-base adhesives used in food-contact materials »

Talanta

Title:		Determination of natural olive fruit fly pheromone in insect samples by enzyme linked immunoassays
Author(s):		Neokosmidi A; Ragoussis V; Zikos C; Paravatou-Petsotas M; Livaniou E; Ragoussis N; Evangelatos G;
Address:		"NCSR Demokritos, RRP Institute, Immunopeptide Chemistry Laboratory, Aghia Paraskevi, 153 10 Athens, Greece"
Journal Title:		Talanta
Year:		2008
Volume:		20070619
Issue:		4
Page Number:		539 - 546
DOI:		10.1016/j.talanta.2007.06.015
ISSN/ISBN:		1873-3573 (Electronic) 0039-9140 (Linking)
Abstract:		"The olive fruit fly pheromone avidin-biotin ELISA immunoassay, based on the use of polyclonal G antibodies derived from rabbits (reported previously) and a newer assay, based on the use of polyclonal Y antibodies isolated from the eggs of laying hens (reported in this paper), were applied successfully for the analysis of natural pheromone in virgin adult female olive fruit flies. According to the results obtained, the pheromone content in the glands of adult female olive fruit flies increases from the third to the ninth day of their age. During the calling period, the female olive fruit flies seem to emit approximately 1.1microg pheromone/insect/day at least. The immunoassay, based on the Y antibodies, is slightly more sensitive (detection limit 40ng/mL) than the assay based on polyclonal anti-pheromone rabbit antiserum (detection limit 80ng/mL). As revealed by thorough cross-reactivity studies, including 14 structurally similar to the olive fruit fly pheromone molecules, the newer immunoassay is less selective than the previous one and seems to cross react with few molecules bearing the spiroketal moiety"
Keywords:		"Animals Cross Reactions Diptera Electrophoresis, Polyacrylamide Gel Enzyme-Linked Immunosorbent Assay/*methods Female Pheromones/*analysis/immunology;"
Notes:		"MedlineNeokosmidi, Afroditi Ragoussis, Valentine Zikos, Christos Paravatou-Petsotas, Maria Livaniou, Evangelia Ragoussis, Nikitas Evangelatos, Gregory eng Validation Study Netherlands 2008/03/29 Talanta. 2008 Jan 15; 74(4):539-46. doi: 10.1016/j.talanta.2007.06.015. Epub 2007 Jun 19"