| Title: | Identification of Pseudomonas aeruginosa and Aspergillus fumigatus mono- and co-cultures based on volatile biomarker combinations |
| Author(s): | Neerincx AH; Geurts BP; Habets MFJ; Booij JA; van Loon J; Jansen JJ; Buydens LMC; van Ingen J; Mouton JW; Harren FJM; Wevers RA; Merkus P; Cristescu SM; Kluijtmans LAJ; |
| Address: | "Department of molecular and laser physics, Institute for Molecules and Materials, Radboud University, Nijmegen, the Netherlands. Department of Paediatrics, Amalia Children's Hospital, Radboud University Medical Center, Nijmegen, the Netherlands. Department of Analytical Chemistry, Institute for Molecules and Materials, Radboud University, Nijmegen, the Netherlands. Department of Medical Microbiology, Radboud University Medical Center, Nijmegen, the Netherlands. Department of Medical Microbiology and Infectious Diseases, Erasmus Medical Center, Rotterdam, the Netherlands. Department of Laboratory Medicine, Translational Metabolic Laboratory, Radboud University Medical Center, Nijmegen, the Netherlands. Department of Paediatrics, Canisius Wilhelmina Hospital, Nijmegen, the Netherlands" |
| DOI: | 10.1088/1752-7155/10/1/016002 |
| ISSN/ISBN: | 1752-7163 (Electronic) 1752-7155 (Linking) |
| Abstract: | "Volatile organic compound (VOC) analysis in exhaled breath is proposed as a non-invasive method to detect respiratory infections in cystic fibrosis patients. Since polymicrobial infections are common, we assessed whether we could distinguish Pseudomonas aeruginosa and Aspergillus fumigatus mono- and co-cultures using the VOC emissions. We took headspace samples of P. aeruginosa, A. fumigatus and co-cultures at 16, 24 and 48 h after inoculation, in which VOCs were identified by thermal desorption combined with gas chromatography - mass spectrometry. Using multivariate analysis by Partial Least Squares Discriminant Analysis we found distinct VOC biomarker combinations for mono- and co-cultures at each sampling time point, showing that there is an interaction between the two pathogens, with P. aeruginosa dominating the co-culture at 48 h. Furthermore, time-independent VOC biomarker combinations were also obtained to predict correct identification of P. aeruginosa and A. fumigatus in mono-culture and in co-culture. This study shows that the VOC combinations in P. aeruginosa and A. fumigatus co-microbial environment are different from those released by these pathogens in mono-culture. Using advanced data analysis techniques such as PLS-DA, time-independent pathogen specific biomarker combinations can be generated that may help to detect mixed respiratory infections in exhaled breath of cystic fibrosis patients" |
| Keywords: | Aspergillus fumigatus/*metabolism Biomarkers/metabolism Coculture Techniques Exhalation Gas Chromatography-Mass Spectrometry Humans Pseudomonas aeruginosa/*metabolism Specimen Handling Volatile Organic Compounds/*analysis; |
| Notes: | "MedlineNeerincx, A H Geurts, B P Habets, M F J Booij, J A van Loon, J Jansen, J J Buydens, L M C van Ingen, J Mouton, J W Harren, F J M Wevers, R A Merkus, P J F M Cristescu, S M Kluijtmans, L A J eng Research Support, Non-U.S. Gov't England 2016/01/30 J Breath Res. 2016 Jan 29; 10(1):016002. doi: 10.1088/1752-7155/10/1/016002" |
