| Title: | Analysis of apocarotenoid volatiles during the development of Ficus carica fruits and characterization of carotenoid cleavage dioxygenase genes |
| Author(s): | Nawade B; Shaltiel-Harpaz L; Yahyaa M; Bosamia TC; Kabaha A; Kedoshim R; Zohar M; Isaacson T; Ibdah M; |
| Address: | "Newe Yaar Research Center, Agricultural Research Organization, P. O. Box 1021, Ramat Yishay, 30095, Israel. Tel Hai College, Upper Galilee 12210, Israel; Migal Galilee Research Institute, P.O. Box 831, Kiryat Shmona, 11016, Israel. ICAR-Directorate of Groundnut Research, P.O. Box 362001, Junagadh, Gujarat, India. Migal Galilee Research Institute, P.O. Box 831, Kiryat Shmona, 11016, Israel. Newe Yaar Research Center, Agricultural Research Organization, P. O. Box 1021, Ramat Yishay, 30095, Israel. Electronic address: mwafaq@volcani.agri.gov.il" |
| DOI: | 10.1016/j.plantsci.2019.110292 |
| ISSN/ISBN: | 1873-2259 (Electronic) 0168-9452 (Linking) |
| Abstract: | "In plants the oxidative cleavage of carotenoid substrates produces volatile apocarotenoids, including beta-ionone, 6-methyl-5-hepten-2-ol, and alpha-ionone; these compounds are important in herbivore-plant communication. Combined chemical, biochemical, and molecular studies were conducted to evaluate the differential accumulation of carotenoids and volatile apocarotenoids during the development of pollinated and parthenocarpic fig fruits. Pollinated fig fruits showed less emission of apocarotenoid volatiles than the parthenocarpic figs, while in the case of carotenoid pigments, pollinated figs manifested higher accumulation. The apocarotenoids, 6-methyl-5-hepten-2-ol and beta-cyclogeraniol, showed a marked increase after the two weeks of hand-pollination in pollinated and parthenocarpic figs; but afterwards these volatile levels decreased during further fruit development. In addition, we report a transcriptome-based identification and functional characterization of the carotenoid cleavage dioxygenase (FcCCD) genes. These genes were overexpressed in Escherichia coli strains previously engineered to produce different carotenoids. The recombinant FcCCD1A enzyme showed specificity for the 9,10 (9',10') double bond position of cyclic carotenoids to generate alpha-ionone and beta-ionone, while FcCCD1B cleaved lycopene and an acyclic moiety of delta-carotene, producing 6-methyl-5-hepten-2-one. The qRT-PCR analysis of FcCCD genes revealed differential gene expression during fig fruit development. Our results suggest a role for the FcCCD1genes in apocarotenoid biosynthesis in fig fruits" |
| Keywords: | Carotenoids/*metabolism Dioxygenases/*metabolism Ficus/enzymology/growth & development/*metabolism Fruit/enzymology/growth & development/metabolism Phylogeny Volatile Organic Compounds/*metabolism 6-methyl-5-hepten-2-ol Apocarotenoid Carotenoid cleavage d; |
| Notes: | "MedlineNawade, Bhagwat Shaltiel-Harpaz, Liora Yahyaa, Mosaab Bosamia, Tejas C Kabaha, Anas Kedoshim, Rika Zohar, Matat Isaacson, Tal Ibdah, Mwafaq eng Ireland 2019/11/30 Plant Sci. 2020 Jan; 290:110292. doi: 10.1016/j.plantsci.2019.110292. Epub 2019 Oct 8" |
