| Title: | Complete genome sequence analysis of a plant growth-promoting phylloplane Bacillus altitudinis FD48 offers mechanistic insights into priming drought stress tolerance in rice |
| Author(s): | Narayanasamy S; Thankappan S; Kumaravel S; Ragupathi S; Uthandi S; |
| Address: | "Biocatalysts Laboratory, Department of Agricultural Microbiology, Tamil Nadu Agricultural University, Coimbatore 641 003, India. Biocatalysts Laboratory, Department of Agricultural Microbiology, Tamil Nadu Agricultural University, Coimbatore 641 003, India. Electronic address: usiva@tnau.ac.in" |
| DOI: | 10.1016/j.ygeno.2022.110550 |
| ISSN/ISBN: | 1089-8646 (Electronic) 0888-7543 (Linking) |
| Abstract: | "Bacillus altitudinis FD48 is a multifunctional plant growth-promoting bacterium isolated from the phylloplane of rice and survives at --10 bars of osmotic potential (--1.0 MPa). It also serves as an ideal PGPM against drought stress by triggering antioxidant defense mechanisms in rice. To further unravel the genetic determinants of osmotic stress tolerance and plant growth-promoting traits, the whole genome sequence of FD48 was compared with its related strains. The whole genome analysis revealed a single chromosome with a total length of 3,752,533 bp (3.7 Mb) and an average G + C ratio of 41.19%. A total of 4029 genes were predicted, of which 3964 (98.4%) were protein-encoding genes (PEGs) and 65 (1.6%) were non-protein-coding genes. The interaction of FD48 with the host plants is associated with many chemotactic and motility-related genes. The ability of FD48 to colonize plants and maintain plant growth under adverse environmental conditions was evidenced by the presence of genes for plant nutrient acquisition, phytohormone synthesis, trehalose, choline, and glycine betaine biosynthesis, microbial volatile organic compounds (acetoin synthesis), heat and cold shock chaperones, translation elongation factor TU (Ef-Tu), siderophore production, DEAD/DEAH boxes, and non- ribosomal peptide synthase clusters (bacilysin, fengycin, and bacitracin). This study sheds light on the drought stress-resilient mechanism, metabolic pathways and potential activity, and plant growth-promoting traits of B. altitudinis FD48 at the genetic level" |
| Keywords: | *Oryza/genetics Droughts *Bacillus/genetics Plants/genetics Sequence Analysis Bacillus altitudinis Drought stress tolerance Phylloplane Secondary metabolites Volatile organic compounds Whole-genome; |
| Notes: | "MedlineNarayanasamy, Shobana Thankappan, Sugitha Kumaravel, Sowmya Ragupathi, Sridar Uthandi, Sivakumar eng Research Support, Non-U.S. Gov't 2022/12/25 Genomics. 2023 Jan; 115(1):110550. doi: 10.1016/j.ygeno.2022.110550. Epub 2022 Dec 21" |
