« Previous AbstractApplication of bioelectrochemical systems to regulate and accelerate the anaerobic digestion processes    Next AbstractChemosensory proteins from the proboscis of mamestra brassicae »

PLoS Genet

Title:		Checkpoints in a yeast differentiation pathway coordinate signaling during hyperosmotic stress
Author(s):		Nagiec MJ; Dohlman HG;
Address:		"Department of Pharmacology, The University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, United States of America"
Journal Title:		PLoS Genet
Year:		2012
Volume:		20120105
Issue:		1
Page Number:		e1002437 -
DOI:		10.1371/journal.pgen.1002437
ISSN/ISBN:		1553-7404 (Electronic) 1553-7390 (Print) 1553-7390 (Linking)
Abstract:		"All eukaryotes have the ability to detect and respond to environmental and hormonal signals. In many cases these signals evoke cellular changes that are incompatible and must therefore be orchestrated by the responding cell. In the yeast Saccharomyces cerevisiae, hyperosmotic stress and mating pheromones initiate signaling cascades that each terminate with a MAP kinase, Hog1 and Fus3, respectively. Despite sharing components, these pathways are initiated by distinct inputs and produce distinct cellular behaviors. To understand how these responses are coordinated, we monitored the pheromone response during hyperosmotic conditions. We show that hyperosmotic stress limits pheromone signaling in at least three ways. First, stress delays the expression of pheromone-induced genes. Second, stress promotes the phosphorylation of a protein kinase, Rck2, and thereby inhibits pheromone-induced protein translation. Third, stress promotes the phosphorylation of a shared pathway component, Ste50, and thereby dampens pheromone-induced MAPK activation. Whereas all three mechanisms are dependent on an increase in osmolarity, only the phosphorylation events require Hog1. These findings reveal how an environmental stress signal is able to postpone responsiveness to a competing differentiation signal, by acting on multiple pathway components, in a coordinated manner"
Keywords:		"Gene Expression Regulation, Fungal MAP Kinase Signaling System/*genetics Mitogen-Activated Protein Kinases/genetics/*metabolism *Osmolar Concentration Pheromones/genetics/*metabolism Phosphorylation Protein Serine-Threonine Kinases/metabolism Saccharomyce;"
Notes:		"MedlineNagiec, Michal J Dohlman, Henrik G eng R01 GM059167/GM/NIGMS NIH HHS/ R01 GM073180/GM/NIGMS NIH HHS/ Research Support, N.I.H., Extramural 2012/01/14 PLoS Genet. 2012 Jan; 8(1):e1002437. doi: 10.1371/journal.pgen.1002437. Epub 2012 Jan 5"