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BMC Genomics

Title:		Comparative transcriptomics of mountain pine beetle pheromone-biosynthetic tissues and functional analysis of CYP6DE3
Author(s):		Nadeau JA; Petereit J; Tillett RL; Jung K; Fotoohi M; MacLean M; Young S; Schlauch K; Blomquist GJ; Tittiger C;
Address:		"Department of Biochemistry and Molecular Biology, University of Nevada, Reno, NV, 89557, USA. Biomedical Engineering Department, University of Nevada, Reno, NV, 89557, USA. Nevada INBRE Bioinformatics Core, University of Nevada, Reno, NV, 89557, USA. Department of Biochemistry and Molecular Biology, University of Nevada, Reno, NV, 89557, USA. crt@unr.edu"
Journal Title:		BMC Genomics
Year:		2017
Volume:		20170420
Issue:		1
Page Number:		311 -
DOI:		10.1186/s12864-017-3696-4
ISSN/ISBN:		1471-2164 (Electronic) 1471-2164 (Linking)
Abstract:		"BACKGROUND: The mountain pine beetle (MPB, Dendroctonus ponderosae Hopkins) is a highly destructive pest of pine forests in western North America. During flight to a new host tree and initiation of feeding, mountain pine beetles release aggregation pheromones. The biosynthetic pathways of these pheromones are sex-specific and localized in the midgut and fat body, but the enzymes involved have not all been identified or characterized. RESULTS: We used a comparative RNA-Seq analysis between fed and unfed male and female MPB midguts and fat bodies to identify candidate genes involved in pheromone biosynthesis. The 13,407 potentially unique transcripts showed clear separation based on feeding state and gender. Gene co-expression network construction and examination using petal identified gene groups that were tightly connected. This, as well as other co-expression and gene ontology analyses, identified all four known pheromone biosynthetic genes, confirmed the tentative identification of four others from a previous study, and suggested nine novel candidates. One cytochrome P450 monooxygenase, CYP6DE3, identified as a possible exo-brevicomin-biosynthetic enzyme in this study, was functionally characterized and likely is involved in resin detoxification rather than pheromone biosynthesis. CONCLUSIONS: Our analysis supported previously characterized pheromone-biosynthetic genes involved in exo-brevicomin and frontalin biosynthesis and identified a number of candidate cytochrome P450 monooxygenases and a putative cyclase for further studies. Functional analyses of CYP6DE3 suggest its role in resin detoxification and underscore the limitation of using high-throughput data to tentatively identify candidate genes. Further functional analyses of candidate genes found in this study should lead to the full characterization of MPB pheromone biosynthetic pathways and the identification of molecular targets for possible pest management strategies"
Keywords:		Animals Coleoptera/enzymology/*genetics/*metabolism Cytochrome P-450 Enzyme System/*genetics/*metabolism *Gene Expression Profiling Gene Ontology Gene Regulatory Networks Pheromones/*biosynthesis Bark beetle Dendroctonus P450 Pheromone RNA-Seq Transcripto;
Notes:		"MedlineNadeau, J A Petereit, J Tillett, R L Jung, K Fotoohi, M MacLean, M Young, S Schlauch, K Blomquist, G J Tittiger, C eng P20 GM103440/GM/NIGMS NIH HHS/ Comparative Study Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. England 2017/04/22 BMC Genomics. 2017 Apr 20; 18(1):311. doi: 10.1186/s12864-017-3696-4"