| Title: | Active conformation of the pyrokinin/PBAN neuropeptide family for pheromone biosynthesis in the silkworm |
| Author(s): | Nachman RJ; Kuniyoshi H; Roberts VA; Holman GM; Suzuki A; |
| Address: | "Food Animal Protection Research Laboratory, U.S. Department of Agriculture, College Station, TX 77845" |
| Journal Title: | Biochem Biophys Res Commun |
| ISSN/ISBN: | 0006-291X (Print) 0006-291X (Linking) |
| Abstract: | "Members of the pyrokinin/pheromone biosynthesis activating neuropeptide family elicit pheromonotropic activity in at least two species of moths. We report that in the silkworm (Bombyx mori) the conformationally constrained octapeptide analog cyclo[Asn-Thr-Ser-Phe-Thr-Pro-Arg-Leu] retains 10% of the pheromonotropic activity of naturally occurring Bom-PBAN-I, a 33 amino acid peptide. Previous data from CD, NMR, and molecular dynamics analyses indicate a type I beta-turn conformation for active core residues Thr-Pro-Arg-Leu in the cyclic analog. The rigidity of the well-defined backbone structure of this cyclic pyrokinin/PBAN analog suggests that it represents the conformation necessary to interact with the pheromonotropic receptor site in the silkworm" |
| Keywords: | "Amino Acid Sequence Animals Bombyx Insect Hormones/*chemistry Models, Molecular Molecular Sequence Data Neuropeptides/*chemistry Peptides, Cyclic/*chemistry Pheromones/*biosynthesis *Protein Conformation Sequence Homology, Amino Acid;" |
| Notes: | "MedlineNachman, R J Kuniyoshi, H Roberts, V A Holman, G M Suzuki, A eng Comparative Study Research Support, U.S. Gov't, Non-P.H.S. 1993/06/15 Biochem Biophys Res Commun. 1993 Jun 15; 193(2):661-6. doi: 10.1006/bbrc.1993.1675" |
