| Title: | Maize Chlorotic Mottle Virus Induces Changes in Host Plant Volatiles that Attract Vector Thrips Species |
| Author(s): | Mwando NL; Tamiru A; Nyasani JO; Obonyo MAO; Caulfield JC; Bruce TJA; Subramanian S; |
| Address: | "International Centre of Insect Physiology and Ecology, P. O. Box 30772-00100, Nairobi, Kenya. Department of Biochemistry and Molecular Biology, Egerton University, P. O. Box 536-20115, Egerton, Kenya. International Centre of Insect Physiology and Ecology, P. O. Box 30772-00100, Nairobi, Kenya. atamiru@icipe.org. Crop Health Unit, Kenya Agricultural and Livestock Research Organization, Embu Research Centre, P. O. Box 27, Embu, 60100, Kenya. Biological Chemistry Department, Rothamsted Research, Harpenden, Herts, AL5 2JQ, UK. School of Life Sciences, Keele University, Keele, Staffordshire, ST5 5BG, UK" |
| DOI: | 10.1007/s10886-018-0973-x |
| ISSN/ISBN: | 1573-1561 (Electronic) 0098-0331 (Linking) |
| Abstract: | "Maize lethal necrosis is one of the most devastating diseases of maize causing yield losses reaching up to 90% in sub-Saharan Africa. The disease is caused by a combination of maize chlorotic mottle virus (MCMV) and any one of cereal viruses in the Potyviridae group such as sugarcane mosaic virus. MCMV has been reported to be transmitted mainly by maize thrips (Frankliniella williamsi) and onion thrips (Thrips tabaci). To better understand the role of thrips vectors in the epidemiology of the disease, we investigated behavioral responses of F. williamsi and T. tabaci, to volatiles collected from maize seedlings infected with MCMV in a four-arm olfactometer bioassay. Volatile profiles from MCMV-infected and healthy maize plants were compared by gas chromatography (GC) and GC coupled mass spectrometry analyses. In the bioassays, both sexes of F. williamsi and male T. tabaci were significantly attracted to volatiles from maize plants infected with MCMV compared to healthy plants and solvent controls. Moreover, volatile analysis revealed strong induction of (E)-4,8-dimethyl-1,3,7-nonatriene, methyl salicylate and (E,E)-4,8,12-trimethyltrideca-1,3,7,11-tetraene in MCMV-infected maize seedlings. Our findings demonstrate MCMV induces changes in volatile profiles of host plants to elicit attraction of thrips vectors. The increased vector contact rates with MCMV-infected host plants could enhance virus transmission if thrips feed on the infected plants and acquire the pathogen prior to dispersal. Uncovering the mechanisms mediating interactions between vectors, host plants and pathogens provides useful insights for understanding the vector ecology and disease epidemiology, which in turn may contribute in designing integrated vector management strategies" |
| Keywords: | Animals Female Gammaherpesvirinae/*physiology Gas Chromatography-Mass Spectrometry *Host-Pathogen Interactions Insect Vectors/virology Male Plant Diseases/*virology Seedlings/chemistry/physiology/virology Thysanoptera/virology Volatile Organic Compounds/a; |
| Notes: | "MedlineMwando, Nelson L Tamiru, Amanuel Nyasani, Johnson O Obonyo, Meshack A O Caulfield, John C Bruce, Toby J A Subramanian, Sevgan eng 2018/06/03 J Chem Ecol. 2018 Aug; 44(7-8):681-689. doi: 10.1007/s10886-018-0973-x. Epub 2018 Jun 2" |
