| Title: | Buffalo nasal odorant-binding protein (bunOBP) and its structural evaluation with putative pheromones |
| Author(s): | Muthukumar S; Rajesh D; Selvam RM; Saibaba G; Suvaithenamudhan S; Akbarsha MA; Padmanabhan P; Gulyas B; Archunan G; |
| Address: | "Center for Pheromone Technology (CPT), Department of Animal Science, Bharathidasan University, Tiruchirappalli, 620024, Tamil Nadu, India. Center for Animal Research, Training and Services (CAReTS), Central Inter-Disciplinary Research Facility (CIDRF), Mahatma Gandhi Medical College & Research Institute campus, Pillaiyarkuppam, Puducherry, 607402, India. Research Institute in Semiochemistry and Applied Ethology (IRSEA), Quartier Salignan, 84400, APT, France. Winro Research Institute of Biological Sciences, winro Science Research Foundation, Tiruchirappalli, 620 007, Tamil Nadu, India. Department of Bioinformatics, Bharathidasan University, Tiruchirappalli, 620024, Tamil Nadu, India. Mahatma Gandhi-Doerenkamp Centre, and Department of Animal Science, Bharathidasan University, Tiruchirappalli, 620024, Tamil Nadu, India. National College (Autonomous), Tiruchirappalli, 620001, Tamil Nadu, India. Lee Kong Chian School of Medicine, Nanyang Technological University, Singapore, 636921, Singapore. ppadmanabhan@ntu.edu.sg. Lee Kong Chian School of Medicine, Nanyang Technological University, Singapore, 636921, Singapore. Center for Pheromone Technology (CPT), Department of Animal Science, Bharathidasan University, Tiruchirappalli, 620024, Tamil Nadu, India. archunan@bdu.ac.in" |
| DOI: | 10.1038/s41598-018-27550-7 |
| ISSN/ISBN: | 2045-2322 (Electronic) 2045-2322 (Linking) |
| Abstract: | "Pheromones are odoriferous volatile chemical cues produced by animals for communication among conspecifics so as to regulate their social behaviors. In general, the odor compounds are recognized by receptors in the nasal cavity. Odorant-binding protein (OBP), a lipocalin family protein, mediates the air-borne odor cues to nasal receptors through nasal mucus. The presence of OBP in several mammalian species is well documented but to-date there is no report of a nasal OBP in buffalo. Hence, the present study was undertaken to investigate if OBP is present in buffalo nasal mucus. Uni- and two-dimensional gel electrophoresis of the nasal mucus suggested the presence of OBP, which was confirmed using mass spectrometry. In silico homology model of the OBP was generated and its structural similarity with other mammalian OBPs was assessed. Finally, molecular-docking and -dynamics simulations analysis revealed the efficiency of buffalo nasal OBP (bunOBP) to bind with buffalo pheromones as well as other reported chemical cues. Taken together, the occurrence of nasal OBP in buffalo and its putative role in odor binding are reported for the first time. The potential association of this protein with estrus-specific volatiles could be taken to advantage for non-invasive detection of estrus in buffaloes" |
| Keywords: | "Animals Buffaloes Electrophoresis, Gel, Two-Dimensional Male Mass Spectrometry Molecular Dynamics Simulation Olfactory Mucosa/*chemistry Pheromones/*chemistry Receptors, Odorant/*chemistry Tandem Mass Spectrometry;" |
| Notes: | "MedlineMuthukumar, Subramanian Rajesh, Durairaj Selvam, Ramu Muthu Saibaba, Ganesan Suvaithenamudhan, Suvaiyarasan Akbarsha, Mohammad Abdulkader Padmanabhan, Parasuraman Gulyas, Balazs Archunan, Govindaraju eng Research Support, Non-U.S. Gov't England 2018/06/21 Sci Rep. 2018 Jun 19; 8(1):9323. doi: 10.1038/s41598-018-27550-7" |
