| Title: | "Efficient production of recombinant human pleiotrophin in yeast, Pichia pastoris" |
| Author(s): | Murasugi A; Kido I; Kumai H; Asami Y; |
| Address: | "Meiji Institute of Health Science, Meiji Milk Products Co., Ltd., Naruda, Odawara, Kanagawa, Japan. AKIRA_MURASUGI@MEIJI-MILK.COM" |
| Journal Title: | Biosci Biotechnol Biochem |
| ISSN/ISBN: | 0916-8451 (Print) 0916-8451 (Linking) |
| Abstract: | "Approximately 260 mg/l of authentic recombinant human pleiotrophin (rhPTN) was expressed into the medium of high-cell density fermentation using a Pichia pastoris protein expression system. The prepro-sequence of yeast alpha-mating factor was used successfully. The recombinant hPTN was efficiently recovered from the medium by expanded bed adsorption, and purified using successive column chromatography steps. In the purified rhPTN preparation, modified rhPTN were scarcely detected. Circular dichroism measurement of the purified PTN showed the presence of the characteristic beta-structures in the protein" |
| Keywords: | "Carrier Proteins/*genetics Cloning, Molecular/*methods Cytokines/*genetics Fermentation Humans Mating Factor Peptides/genetics Pichia/*genetics Protein Structure, Secondary Recombinant Proteins/biosynthesis/chemistry/isolation & purification Transgenes;" |
| Notes: | "MedlineMurasugi, Akira Kido, Isao Kumai, Hideshi Asami, Yukio eng England 2003/10/31 Biosci Biotechnol Biochem. 2003 Oct; 67(10):2288-90. doi: 10.1271/bbb.67.2288" |
