| Title: | Desorption Electrospray Ionization Mass Spectrometry: 20 Years |
| Address: | "Department of Chemistry and Bindley Bioscience Center, Purdue University, West Lafayette, Indiana 47907, United States" |
| DOI: | 10.1021/acs.accounts.3c00382 |
| ISSN/ISBN: | 1520-4898 (Electronic) 0001-4842 (Linking) |
| Abstract: | "ConspectusMass spectrometry (MS) is one of the most widely used technologies in the chemical sciences. With applications spanning the monitoring of reaction products, the identification of disease biomarkers, and the measurement of thermodynamic parameters and aspects of structural biology, MS is well established as a universal analytical tool applicable to small compounds as well as large molecular complexes. Regardless of the application, the generation of gas-phase ions from neutral compounds is a key step in any MS experiment. However, this ionization step was for many years limited to high-energy approaches that required gas-phase analytes and thus it was restricted to volatile samples. Over the last few decades, new methodologies have been developed to address this limitation and facilitate ionization of biological molecules. Electrospray ionization (ESI) is the most broadly used of these methods, as it facilitates the ionization of intact polar compounds from solution.Twenty years ago, our group reported a new ionization method that uses a charged solvent spray to impact a surface, generating ions from objects rather than just solutions and doing so directly in the ambient environment with no vacuum requirements and little to no sample preparation. This method was termed desorption electrospray ionization (DESI), and it initiated a new field that would come to be known as ambient mass spectrometry. The simplicity and wide applicability of the DESI technology horizontal line and the tens of ambient ionization methods developed subsequently horizontal line revolutionized the MS analysis of complex materials for their organic components, especially for in situ applications.This Account describes the history of DESI, starting with the development of the technique from early electrosonic spray ionization (ESSI) experimental observations as well as the studies leading to the understanding of its mechanism as a 'droplet pick-up' phenomenon involving sequential events (i.e., thin film formation, solid-liquid extraction, secondary droplet generation, and ESI-like ionization from these droplets). We also overview the developments and applications of the technology that have been demonstrated by our group during the last two decades. In particular, we describe (i) the use of DESI for tissue imaging, one of its more significant applications to date, and its extension to intraoperative clinical diagnosis; (ii) the integration of the technology with portable instrumentation for in situ analysis, especially when coupled with tandem mass spectrometry (MS/MS); (iii) the use of DESI microdroplets as microvessels to accelerate organic reactions by orders of magnitude compared to those in bulk solution; and (iv) the combination of all these capabilities for automated high-throughput experiments aimed at accelerating drug discovery" |
| Keywords: | "Drug Discovery *Pentaerythritol Tetranitrate Solvents *Spectrometry, Mass, Electrospray Ionization Tandem Mass Spectrometry;" |
| Notes: | "MedlineMorato, Nicolas M Cooks, R Graham eng Review 2023/09/06 Acc Chem Res. 2023 Sep 19; 56(18):2526-2536. doi: 10.1021/acs.accounts.3c00382. Epub 2023 Sep 6" |
