| Title: | Investigation of sweat VOC profiles in assessment of cancer biomarkers using HS-GC-MS |
| Author(s): | Monedeiro F; Dos Reis RB; Peria FM; Sares CTG; De Martinis BS; |
| Address: | "Department of Chemistry, Faculty of Philosophy, Sciences and Letters of Ribeirao Preto, University of Sao Paulo, Ribeirao Preto, CEP 14040-901, Brazil" |
| ISSN/ISBN: | 1752-7163 (Electronic) 1752-7155 (Linking) |
| Abstract: | "Volatile organic compounds (VOCs) have been studied in biological samples in order to be related to the presence of diseases. Sweat can represent substances existing in blood, has less complex composition (compared with other biological matrices) and can be obtained in a non-invasive way. In this work, sweat patches were collected from healthy controls and volunteers with cancer. Static Headspace was used for VOCs extraction, analysis was performed by gas chromatography coupled with mass spectrometry. Principal Components Analysis was used to investigate data distribution. Random Forest was employed to develop classificatory models. Controls and positive cases could be distinguished with maximum sensitivity and specificity (100% of accuracy) in a model based on the incidence of 2-ethyl-1-hexanol, hexanal and octanal. Discrimination between controls, primary tumors and metastasis was achieved using a panel with 11 VOCs. Balanced accuracy of more than 70% was obtained for the classification of a neoplasm site. Total n-aldehydes presented to be strongly correlated with staging of adenocarcinomas, while phenol and 2,6-dimethyl-7-octen-2-ol were correlated with Gleason score. These findings corroborate with the development of accessible screening tools based on VOC analysis and highlight sweat as a promising matrix to be studied in a clinical context for cancer diagnosis" |
| Keywords: | "Biomarkers, Tumor/*analysis Breath Tests Case-Control Studies Female Gas Chromatography-Mass Spectrometry/*methods Humans Male Middle Aged Principal Component Analysis ROC Curve Solid Phase Microextraction/methods Sweat/*chemistry Temperature Volatile Org;" |
| Notes: | "MedlineMonedeiro, Fernanda Dos Reis, Rodolfo Borges Peria, Fernanda Maris Sares, Claudia Tarcila Gomes De Martinis, Bruno Spinosa eng Research Support, Non-U.S. Gov't England 2019/11/26 J Breath Res. 2020 Feb 25; 14(2):026009. doi: 10.1088/1752-7163/ab5b3c" |
