| Title: | "Isolation, endocrine regulation and mRNA distribution of the 3-hydroxy-3-methylglutaryl coenzyme A synthase (HMG-S) gene from the pine engraver, Ips pini (Coleoptera: Scolytidae)" |
| Author(s): | Bearfield JC; Keeling CI; Young S; Blomquist GJ; Tittiger C; |
| Address: | "Department of Biochemistry and Molecular Biology, University of Nevada, Reno, 89557, USA" |
| DOI: | 10.1111/j.1365-2583.2006.00627.x |
| ISSN/ISBN: | 0962-1075 (Print) 0962-1075 (Linking) |
| Abstract: | "We isolated a full-length cDNA encoding 3-hydroxy-3-methylglutaryl coenzyme A synthase (HMG-S) from the pine engraver beetle, Ips pini (Say), and examined its genomic structure. The intron-less gene has a predicted 460 amino acid cytosolic protein product with 73% identity to HMG-S from Dendroctonus jeffreyi, and high identity (58-64%) with other insect HMG-Ss. Topically applied juvenile hormone (JH) III induced HMG-S mRNA levels up to 6.5-fold in both sexes, mostly in the anterior midgut, though there were differences between males and females in the timing, sensitivity to JH III dose and tissue distribution of HMG-S mRNA. These data further validate the coordinate regulation of mevalonate pathway genes for de novo isoprenoid pheromone production in bark beetles" |
| Keywords: | "Amino Acid Sequence Animals Base Sequence Coleoptera/*enzymology/genetics Female Gene Expression Genes, Insect Hydroxymethylglutaryl-CoA Synthase/*genetics/metabolism Male Molecular Sequence Data Pheromones/biosynthesis RNA, Messenger/metabolism Sequence;" |
| Notes: | "MedlineBearfield, J C Keeling, C I Young, S Blomquist, G J Tittiger, C eng P20 RR 16464/RR/NCRR NIH HHS/ Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. England 2006/04/28 Insect Mol Biol. 2006 Apr; 15(2):187-95. doi: 10.1111/j.1365-2583.2006.00627.x" |
