| Title: | Breathprints of model murine bacterial lung infections are linked with immune response |
| Author(s): | Bean HD; Jimenez-Diaz J; Zhu J; Hill JE; |
| Address: | "Thayer School of Engineering, Dartmouth College, Hanover, NH, USA These authors contributed equally to this study. School of Engineering, University of Vermont, Burlington, VT, USA These authors contributed equally to this study. School of Engineering, University of Vermont, Burlington, VT, USA. Thayer School of Engineering, Dartmouth College, Hanover, NH, USA Jane.Hill@Dartmouth.edu" |
| DOI: | 10.1183/09031936.00015814 |
| ISSN/ISBN: | 1399-3003 (Electronic) 0903-1936 (Print) 0903-1936 (Linking) |
| Abstract: | "In this model study, we explored the host's contribution of breath volatiles to diagnostic secondary electrospray ionisation-mass spectrometry (SESI-MS) breathprints for acute bacterial lung infections, their correlation with the host's immune response, and their use in identifying the lung pathogen. Murine airways were exposed to Pseudomonas aeruginosa and Staphylococcus aureus bacterial cell lysates or to PBS (controls), and their breath and bronchoalveolar lavage fluid (BALF) were collected at six time points (from 6 to 120 h) after exposure. Five to six mice per treatment group and four to six mice per control group were sampled at each time. Breath volatiles were analysed using SESI-MS and the BALF total leukocytes, polymorphonuclear neutrophils, lactate dehydrogenase activity, and cytokine concentrations were quantified. Lysate exposure breathprints contain host volatiles that persist for up to 120 h; are pathogen specific; are unique from breathprints of controls, active infections and cleared infections; and are correlated with the host's immune response. Bacterial lung infections induce changes to the host's breath volatiles that are selective and specific predictors of the source of infection. Harnessing the pathogen-specific volatiles in the host's breath may provide useful information for detecting latent bacterial lung infections and managing the spread of respiratory diseases" |
| Keywords: | "Animals Bacterial Infections/immunology Breath Tests Bronchoalveolar Lavage Fluid/immunology Cytokines/immunology Discriminant Analysis Disease Models, Animal L-Lactate Dehydrogenase/metabolism Least-Squares Analysis Lung/immunology Lung Diseases/*diagnos;" |
| Notes: | "MedlineBean, Heather D Jimenez-Diaz, Jaime Zhu, Jiangjiang Hill, Jane E eng P20 GM103496/GM/NIGMS NIH HHS/ P20 RR021905/RR/NCRR NIH HHS/ 8 P20 GM103496-07/GM/NIGMS NIH HHS/ 5P20RR021905-07/RR/NCRR NIH HHS/ Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. England 2014/10/18 Eur Respir J. 2015 Jan; 45(1):181-90. doi: 10.1183/09031936.00015814. Epub 2014 Oct 16" |
