« Previous AbstractIonotropic glutamate receptors mediate inducible defense in the water flea Daphnia pulex    Next Abstract"Biosynthesis and secretion of tremerogen A-10, a polyisoprenyl peptide mating pheromone of Tremella mesenterica" »

J Bacteriol

Title:		Mating pheromone-induced alteration of cell surface proteins in the heterobasidiomycetous yeast Tremella mesenterica
Author(s):		Miyakawa T; Kadota T; Okubo Y; Hatano T; Tsuchiya E; Fukui S;
Address:
Journal Title:		J Bacteriol
Year:		1984
Volume:		158
Issue:		3
Page Number:		814 - 819
DOI:		10.1128/jb.158.3.814-819.1984
ISSN/ISBN:		0021-9193 (Print) 1098-5530 (Electronic) 0021-9193 (Linking)
Abstract:		"Mating pheromone-induced alteration of the cell surface proteins of haploid cells, presumed to play crucial roles in the specific cell-cell interactions during sexual conjugation of Tremella mesenterica , was investigated. Exposed surface proteins were revealed by lactoperoxidase-catalyzed iodination in combination with polyacrylamide gel electrophoresis and autoradiography. From comparison of the molecular species of 125I-labeled surface proteins of the vegetative and the gamete (mating pheromone-treated) cells of the two compatible mating types (ab and AB), it was suggested that a striking change in cell surface structure occurs during the differentiation; although labeled protein species of the vegetative cells of the two mating types were indistinguishable, several new species, both mating type specific and nonspecific, appeared in the gamete cells. Turnover of the labeled proteins of the vegetative cells was negligible, whereas that of the gamete cells was rapid with release of low-molecular-weight labeled proteins in the medium. A role for the labeled surface proteins of the gamete cells in the cell-cell interactions during sexual conjugation was suggested by the following: the surface changes were induced by mating pheromone; the labeled proteins were preferentially localized on the surface of the mating tube; the labeled species appeared sequentially during the differentiation; and mating type-specific species were present in both mating types"
Keywords:		"Animals Basidiomycota/*physiology Cell Membrane/physiology Electrophoresis, Polyacrylamide Gel Haploidy Kinetics Mating Factor Membrane Proteins/isolation & purification/*physiology Microscopy, Electron Molecular Weight Peptides/*physiology Pheromones/*ph;"
Notes:		"MedlineMiyakawa, T Kadota, T Okubo, Y Hatano, T Tsuchiya, E Fukui, S eng Research Support, Non-U.S. Gov't 1984/06/01 J Bacteriol. 1984 Jun; 158(3):814-9. doi: 10.1128/jb.158.3.814-819.1984"