| Title: | Identification of Lathyrus sativus plant volatiles causing behavioral preference of Aphis craccivora |
| Author(s): | Mitra P; Das S; Debnath R; Mobarak SH; Barik A; |
| Address: | "Ecology Research Laboratory, Department of Zoology, The University of Burdwan, Burdwan, India" |
| ISSN/ISBN: | 1526-4998 (Electronic) 1526-498X (Linking) |
| Abstract: | "BACKGROUND: The viviparous aphid Aphis craccivora Koch (Hemiptera: Aphididae) is a serious threat to the crop yield of Lathyrus sativus L. (Fabaceae), commonly known as grass pea. The synthetic insecticides applied to control this insect pest are not safe for the environment. Hence, it is necessary to find volatile organic compounds (VOCs) from two cultivars [BIO L 212 Ratan (BIO) and Nirmal B-1 (NIR)] of L. sativus plants causing behavioral preference of A. craccivora. RESULTS: The VOCs from undamaged (UD), insect-damaged (ID) [plants on which 50 or 100 adults of A. craccivora were fed for 4 h (ID 50 or ID 100)], and mechanically damaged (MD) plants were identified and quantified by gas chromatography-mass spectrometry and gas chromatography-flame ionization detection analyses, respectively. Total VOCs were higher in ID plants compared to UD plants of each cultivar. However, total VOCs were higher in NIR cultivar compared to BIO cultivar for both UD and ID plants. Benzyl alcohol was predominant in volatile extracts of all treatments. In Y-tube olfactometer bioassays, females showed preference towards volatile extracts of UD, ID, and MD plants of each cultivar compared to the control solvent (CH(2) Cl(2) ). Insects preferred certain synthetic blends comparable to volatile extracts of UD, ID, and MD plants of each L. sativus cultivar against the control solvent. CONCLUSION: Females preferred a synthetic blend of benzyl alcohol, 1,3-diethylbenzene, thymol, and 1-hexadecene at ratios of 142.49: 62.03:1.18:1 dissolved in 25 muL of CH(2) Cl(2) in olfactometer bioassays, which could be used in developing lures to control this insect pest" |
| Keywords: | "Animals *Aphids Female Gas Chromatography-Mass Spectrometry *Lathyrus *Volatile Organic Compounds/analysis 1, 3-diethylbenzene 1-hexadecene Aphididae Aphis craccivora Hemiptera Lathyrus sativus benzyl alcohol olfactometer bioassay plants thymol volatiles;" |
| Notes: | "MedlineMitra, Paroma Das, Swati Debnath, Rahul Mobarak, Syed Husne Barik, Anandamay eng DST-SERB, New Delhi, Govt. of India/ England 2020/07/23 Pest Manag Sci. 2021 Jan; 77(1):285-299. doi: 10.1002/ps.6018. Epub 2020 Aug 16" |
