| Title: | Construction of a Biohybrid Odorant Sensor Using Biological Olfactory Receptors Embedded into Bilayer Lipid Membrane on a Chip |
| Author(s): | Misawa N; Fujii S; Kamiya K; Osaki T; Takaku T; Takahashi Y; Takeuchi S; |
| Address: | "Kanagawa Institute of Industrial Science and Technology , Kawasaki , Kanagawa 213-0012 , Japan. Institute of Industrial Science , The University of Tokyo , Meguro , Tokyo 153-8505 , Japan. Environmental Health Science Laboratory , Sumitomo Chemical Company, Limited , Konohana , Osaka 554-8558 , Japan" |
| DOI: | 10.1021/acssensors.8b01615 |
| ISSN/ISBN: | 2379-3694 (Electronic) 2379-3694 (Linking) |
| Abstract: | "This paper describes an odorant sensor based on mosquito olfactory receptors (ORs) that is sensitive to the volatile organic compound octenol. The ORs and OR coreceptors were reconstructed in the lipid bilayer membrane in a chamber device equipped with electrodes. Using this odorant sensor, we obtained ion current changes caused by specific OR responses to octenol. We installed the odorant sensor into a mobile robot and succeeded in the demonstration of coupling octenol gas detection and robot actuation. We believe that this biohybrid odorant sensing system will be a key technology for future artificial olfaction" |
| Keywords: | "Animals Biosensing Techniques/*instrumentation Cell Membrane/*metabolism Electrodes Equipment Design Lipid Bilayers/*metabolism Octanols/analysis Odorants/*analysis Receptors, Odorant/*metabolism Robotics Sf9 Cells Spodoptera bilayer lipid membrane mobile;" |
| Notes: | "MedlineMisawa, Nobuo Fujii, Satoshi Kamiya, Koki Osaki, Toshihisa Takaku, Tomoyuki Takahashi, Yasuhiko Takeuchi, Shoji eng 2019/03/05 ACS Sens. 2019 Mar 22; 4(3):711-716. doi: 10.1021/acssensors.8b01615. Epub 2019 Mar 13" |
