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Metabolites

Title:		Metabolic Profiling of PGPR-Treated Tomato Plants Reveal Priming-Related Adaptations of Secondary Metabolites and Aromatic Amino Acids
Author(s):		Mhlongo MI; Piater LA; Steenkamp PA; Labuschagne N; Dubery IA;
Address:		"Department of Biochemistry, University of Johannesburg, Auckland Park, Johannesburg 2006, South Africa. Department of Plant and Soil Sciences, University of Pretoria, Hatfield, Pretoria 0028, South Africa"
Journal Title:		Metabolites
Year:		2020
Volume:		20200520
Issue:		5
Page Number:		-
DOI:		10.3390/metabo10050210
ISSN/ISBN:		2218-1989 (Print) 2218-1989 (Electronic) 2218-1989 (Linking)
Abstract:		"Plant growth-promoting rhizobacteria (PGPR) are beneficial microbes in the rhizosphere that can directly or indirectly stimulate plant growth. In addition, some can prime plants for enhanced defense against a broad range of pathogens and insect herbivores. In this study, four PGPR strains (Pseudomonas fluorescens N04, P. koreensis N19, Paenibacillus alvei T19, and Lysinibacillus sphaericus T22) were used to induce priming in Solanum lycopersicum (cv. Moneymaker) plants. Plants were inoculated with each of the four PGPRs, and plant tissues (roots, stems, and leaves) were harvested at 24 h and 48 h post-inoculation. Methanol-extracted metabolites were analyzed by ultra-high performance liquid chromatography mass spectrometry (UHPLC-MS). Chemometric methods were applied to mine the data and characterize the differential metabolic profiles induced by the PGPR. The results revealed that all four strains induced defense-related metabolic reprogramming in the plants, characterized by dynamic changes to the metabolomes involving hydroxycinnamates, benzoates, flavonoids, and glycoalkaloids. In addition, targeted analysis of aromatic amino acids indicated differential quantitative increases or decreases over a two-day period in response to the four PGPR strains. The metabolic alterations point to an altered or preconditioned state that renders the plants primed for enhanced defense responses. The results contribute to ongoing efforts in investigating and unraveling the biochemical processes that define the PGPR priming phenomenon"
Keywords:		chemometrics metabolic reprogramming metabolomics plant defense plant growth-promoting rhizobacteria priming;
Notes:		"PubMed-not-MEDLINEMhlongo, Msizi I Piater, Lizelle A Steenkamp, Paul A Labuschagne, Nico Dubery, Ian A eng Switzerland 2020/05/24 Metabolites. 2020 May 20; 10(5):210. doi: 10.3390/metabo10050210"