| Title: | Development of a bisphenol A-adsorbing yeast by surface display of the Kluyveromyces yellow enzyme on Pichia pastoris |
| Author(s): | Mergler M; Wolf K; Zimmermann M; |
| Address: | "Department of Microbiology, Aachen University of Technology, Worringer Weg 1, 52056 Aachen, Germany" |
| Journal Title: | Appl Microbiol Biotechnol |
| DOI: | 10.1007/s00253-003-1361-0 |
| ISSN/ISBN: | 0175-7598 (Print) 0175-7598 (Linking) |
| Abstract: | A novel surface-engineered strain of yeast Pichia pastoris was constructed that displays at its surface Kluyveromyces lactis Yellow Enzyme (KYE) fused to the C-terminal half of Saccharomyces cerevisiae alpha-agglutinin. The expression of the fusion protein was controlled by the AOX1-promoter. The new strain showed an increased sorption of the xenoestrogen Bisphenol A (BPA). It was shown that sorption of BPA depended on the presence of methanol in the growth medium and on the pH of the binding assays. The binding kinetics were typical for binding at a surface. The present results demonstrate that the alpha-agglutinin surface display system can be used in the yeast P. pastoris |
| Keywords: | "Adsorption Alcohol Oxidoreductases/genetics Artificial Gene Fusion Benzhydryl Compounds Biodegradation, Environmental Culture Media/chemistry Fungal Proteins/genetics/metabolism Gene Expression Regulation, Fungal Genes, Fungal Hydrogen-Ion Concentration K;" |
| Notes: | "MedlineMergler, M Wolf, K Zimmermann, M eng Research Support, Non-U.S. Gov't Germany 2003/06/13 Appl Microbiol Biotechnol. 2004 Jan; 63(4):418-21. doi: 10.1007/s00253-003-1361-0. Epub 2003 Jun 12" |
