| Title: | Analysis of apocarotenoid volatiles from lettuce (Lactuca sativa) induced by insect herbivores and characterization of carotenoid cleavage dioxygenase gene |
| Author(s): | Meng K; Eldar-Liebreich M; Nawade B; Yahyaa M; Shaltiel-Harpaz L; Coll M; Sadeh A; Ibdah M; |
| Address: | "Plant Sciences Institute, Newe Yaar Research Center, Agricultural Research Organization (ARO), Ramat Yishay, 30095 Israel. GRID: grid.410498.0. ISNI: 0000 0001 0465 9329 Present Address: Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing, China. GRID: grid.410727.7. ISNI: 0000 0001 0526 1937 Agroecology Lab, Department of Natural Resources, Newe Yaar Research Center, ARO, Ramat Yishay, 30095 Israel. GRID: grid.410498.0. ISNI: 0000 0001 0465 9329 Department of Entomology, Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot , 7610001 Israel. GRID: grid.9619.7. ISNI: 0000 0004 1937 0538 Migal Galilee Research Institute, P.O. Box 831, 11016 Qiryat Shemona, Israel. GRID: grid.425662.1. ISNI: 0000 0004 0404 5732" |
| DOI: | 10.1007/s13205-023-03511-4 |
| ISSN/ISBN: | 2190-572X (Print) 2190-5738 (Electronic) 2190-5738 (Linking) |
| Abstract: | "Plant apocarotenoids have been shown to have a diverse biological role in herbivore-plant interactions. Despite their importance, little is known about herbivores' effect on apocarotenoid emissions in Lactuca sativa. In this study, we examined changes in apocarotenoid emissions in lettuce leaves after infestation by two insects, viz., Spodoptera littoralis larvae and Myzus persicae aphids. We found that beta-ionone and beta-cyclocitral showed higher concentrations than the other apocarotenoids, with a significant increase as per the intensity of infestation of both herbivore species. Furthermore, we performed functional characterization of Lactuca sativa carotenoid cleavage dioxygenase 1 (LsCCD1) genes. Three LsCCD1 genes were overexpressed in E. coli strains, and recombinant proteins were assayed for cleavage activity on an array of carotenoid substrates. The LsCCD1 protein cleaved beta-carotene at the 9,10 (9',10') positions producing beta-ionone. The transcript analysis of LsCCD1 genes revealed differential expression patterns under varying levels of herbivores' infestation, but the results were inconsistent with the pattern of beta-ionone concentrations. Our results suggest that LsCCD1 is involved in the production of beta-ionone, but other regulatory factors might be involved in its induction in response to herbivory. These results provide new insights into apocarotenoid production in response to insect herbivory in lettuce. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s13205-023-03511-4" |
| Keywords: | Apocarotenoid Carotenoid cleavage dioxygenase Insect herbivory Lactuca sativa beta-Ionone; |
| Notes: | "PubMed-not-MEDLINEMeng, Kun Eldar-Liebreich, Michal Nawade, Bhagwat Yahyaa, Mosaab Shaltiel-Harpaz, Liora Coll, Moshe Sadeh, Asaf Ibdah, Mwafaq eng Germany 2023/02/28 3 Biotech. 2023 Mar; 13(3):94. doi: 10.1007/s13205-023-03511-4. Epub 2023 Feb 22" |
