| Title: | Geographical origin of Chinese wolfberry (goji) determined by carbon isotope analysis of specific volatile compounds |
| Author(s): | Meng J; Liu Z; Gou CL; Rogers KM; Yu WJ; Zhang SS; Yuan YW; Zhang L; |
| Address: | "Instrumental Analysis Center, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, China; School of Pharmacy, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, China. Institute of Quality and Standard for Agro-products, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China; Key Laboratory of Information Traceability for Agricultural Products, Ministry of Agriculture and Rural affairs, Hangzhou 310021, China. Institute of Agricultural Product Quality Standard and Testing Technology of Ningxia, Yinchuan 750002, China. National Isotope Centre, GNS Science, 30 Gracefield Road, Lower Hutt 5040, New Zealand. Instrumental Analysis Center, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, China. Institute of Quality and Standard for Agro-products, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China; Key Laboratory of Information Traceability for Agricultural Products, Ministry of Agriculture and Rural affairs, Hangzhou 310021, China. Electronic address: yuanyw@mail.zaas.ac.cn. Instrumental Analysis Center, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, China. Electronic address: lizhang09@sjtu.edu.cn" |
| Journal Title: | J Chromatogr B Analyt Technol Biomed Life Sci |
| DOI: | 10.1016/j.jchromb.2018.12.011 |
| ISSN/ISBN: | 1873-376X (Electronic) 1570-0232 (Linking) |
| Abstract: | "Chinese wolfberry or goji berry (Lycium barbarum) is an important traditional Chinese medicine. Its price and function has a close correlation with its geographical provenance. Illegal mislabeling motivated by commercial gains brings serious food safety problems and damages consumer confidence. In this work, a novel analytical strategy combined with chemometrics statistic tools was developed to determine the geographical origin of wolfberries from different provinces in China. Stable carbon isotopic ratios (delta(13)C) of wolfberry volatile compounds (i.e. limonene, tetramethylpyrazine, safranal, geranylacetone, and beta-ionone) were determined by gas chromatography-combustion-isotope ratio mass spectrometry (GC-IRMS) with headspace-solid phase micro extraction (HS-SPME). Five types of SPME fiber (i.e. DVB/CAR/PDMS, CAR/PDMS, PDMS/DVB?ª++?ª+OC, PDMS, and PA), extraction time, temperature and GC-IRMS conditions were comprehensively optimized to obtain the best adsorption of volatile compounds in wolfberry. Method integrity was assessed by comparing volatiles extracted using HS-SPME GC-IRMS with direct injection GC-IRMS and were in good agreement with each other. The geographical variations of volatile compounds using one-way analysis of variance (ANOVA) were explored for individual delta(13)C values in wolfberry samples from Gansu, Ningxia and Qinghai. Geographical origin of wolfberry was differentiated by linear discrimination analysis (LDA), with an accuracy of 89.16%, 87.77% and 85.87% for these three provinces, respectively. These results showed the combination of SPME and IRMS provides a rapid and valid method to determine the geographical origin of wolfberry" |
| Keywords: | Carbon Isotopes/*analysis China Discriminant Analysis Gas Chromatography-Mass Spectrometry/methods Lycium/*chemistry/*classification Plant Extracts/analysis/*chemistry Reproducibility of Results Solid Phase Microextraction/methods Volatile Organic Compoun; |
| Notes: | "MedlineMeng, Jing Liu, Zhi Gou, Chun-Lin Rogers, Karyne M Yu, Wen-Juan Zhang, Shan-Shan Yuan, Yu-Wei Zhang, Li eng Netherlands 2018/12/24 J Chromatogr B Analyt Technol Biomed Life Sci. 2019 Jan 15; 1105:104-112. doi: 10.1016/j.jchromb.2018.12.011. Epub 2018 Dec 11" |
