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Cell

Title:		"Dual regulation of the yeast CDC28-p40 protein kinase complex: cell cycle, pheromone, and nutrient limitation effects"
Author(s):		Mendenhall MD; Jones CA; Reed SI;
Address:
Journal Title:		Cell
Year:		1987
Volume:		50
Issue:		6
Page Number:		927 - 935
DOI:		10.1016/0092-8674(87)90519-8
ISSN/ISBN:		0092-8674 (Print) 0092-8674 (Linking)
Abstract:		"A 40 kd polypeptide that coprecipitates with the CDC28 gene product in immune complexes is specifically phosphorylated by the CDC28 protein kinase. Using this reaction, we detect activity only in extracts from dividing G1 phase cells. Exit from G1 by entry into S phase or the preconjugatory state induced by mating pheromone correlates with loss of p40 phosphorylation activity. Inactive extracts from cdc28 mutants complement extracts from cells arrested in S or M phase, suggesting that non-G1 cells are deficient in an exchangeable activating factor. Stationary and pheromone-treated cultures are rich in this exchangeable factor, but possess an inactive kinase that is not activated by complementation. cAMP-deficient mutants resemble stationary cells"
Keywords:		"Adenylyl Cyclases/physiology Benzimidazoles/pharmacology Cell Cycle/drug effects Cyclic AMP/physiology Cyclin-Dependent Kinase Inhibitor Proteins Enzyme Activation Fungal Proteins/*physiology Hydroxyurea/pharmacology Mating Factor Models, Biological Nocod;"
Notes:		"MedlineMendenhall, M D Jones, C A Reed, S I eng GM28005/GM/NIGMS NIH HHS/ GM38328/GM/NIGMS NIH HHS/ Comparative Study Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. 1987/09/11 Cell. 1987 Sep 11; 50(6):927-35. doi: 10.1016/0092-8674(87)90519-8"