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Parasitol Res

Title:		Early developmental stages of Ascaris lumbricoides featured by high-resolution mass spectrometry
Author(s):		Melo CF; Esteves CZ; de Oliveira RN; Guerreiro TM; de Oliveira DN; Lima EO; Mine JC; Allegretti SM; Catharino RR;
Address:		"Innovare Biomarkers Laboratory, School of Pharmaceutical Sciences, University of Campinas, Campinas, Sao Paulo, Brazil. Biology Institute, Animal Biology Department, University of Campinas, Campinas, Sao Paulo, Brazil. Department of Clinical and Toxicological Analysis, State University of Ponta Grossa- UEPG, Ponta Grossa, Parana, Brazil. Biology Institute, Animal Biology Department, University of Campinas, Campinas, Sao Paulo, Brazil. sallegre@unicamp.br. Innovare Biomarkers Laboratory, School of Pharmaceutical Sciences, University of Campinas, Campinas, Sao Paulo, Brazil. rrc@fcm.unicamp.br"
Journal Title:		Parasitol Res
Year:		2016
Volume:		20160713
Issue:		11
Page Number:		4107 - 4114
DOI:		10.1007/s00436-016-5183-2
ISSN/ISBN:		1432-1955 (Electronic) 0932-0113 (Linking)
Abstract:		"Ascaris lumbricoides is responsible for a highly disseminated helminth parasitic disease, ascariosis, a relevant parasitosis that responds for great financial burden on the public health system of developing countries. In this work, metabolic fingerprinting using high-resolution mass spectrometry (HRMS) was employed to identify marker molecules from A. lumbricoides in different development stages. We have identified nine biomarkers, such as pheromones and steroidal prohormones in early stages, among other molecules in late development stages, making up four molecules for fertilized eggs, four marker molecules for first larvae (L1) and one marker molecule for third larvae (L3). Therefore, our findings indicate that this approach is suitable for biochemical characterization of A. lumbricoides development stages. Moreover, the straightforward analytical method employed, with almost no sample preparation from a complex matrix (feces) using high-resolution mass spectrometry, suggests that it is possible to seek for an easier and faster way to study animal molding processes"
Keywords:		Animals Ascariasis/*parasitology Ascaris lumbricoides/*growth & development/metabolism Biomarkers/analysis/metabolism Feces/parasitology Female Humans Larva Male Mass Spectrometry/*methods *Metabolomics Ascaris lumbricoides Mass spectrometry Metabolomics;
Notes:		"MedlineMelo, Carlos Fernando Odir Rodrigues Esteves, Cibele Zanardi de Oliveira, Rosimeire Nunes Guerreiro, Tatiane Melina de Oliveira, Diogo Noin Lima, Estela de Oliveira Mine, Julio Cesar Allegretti, Silmara Marques Catharino, Rodrigo Ramos eng Germany 2016/07/15 Parasitol Res. 2016 Nov; 115(11):4107-4114. doi: 10.1007/s00436-016-5183-2. Epub 2016 Jul 13"