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Biol Pharm Bull

Title:		Decreases in pheromonal responses at the accessory olfactory bulb of mice with a deficiency of the alpha1B or beta3 subunits of voltage-dependent Ca2+-channels
Author(s):		Murakami M; Matsui H; Shiraiwa T; Suzuki T; Sasano H; Takahashi E; Kashiwayanagi M;
Address:		"Department of Pharmacology, Akita University School of Medicine, Japan"
Journal Title:		Biol Pharm Bull
Year:		2006
Volume:		29
Issue:		3
Page Number:		437 - 442
DOI:		10.1248/bpb.29.437
ISSN/ISBN:		0918-6158 (Print) 0918-6158 (Linking)
Abstract:		"Pheromones affect gonadal functions and sexual behaviors. Information in regard to pheromones is received by the vomeronasal organ (VNO) and transmitted to the accessory olfactory bulb (AOB). We investigated the physiological role of the alpha1B and beta3 subunits of the N (neuronal)-type voltage-dependent Ca2+ channel in the neurotransduction in the accessory olfactory (vomeronasal) system using alpha1B-deficient mice and beta3-deficient mice. RT-PCR studies showed the existence of beta1, beta2, beta3, beta4, alpha1A, alpha1B, and alpha1C subunits of voltage-dependent Ca2+ channels in the mouse VNO. Immunohistochemical studies showed that the alpha1A, alpha1B, and alpha1C subunits of voltage-dependent Ca2+ channels exist in the sensory neurons and supporting cells of the mouse VNO. Exposure of the VNO to urine samples excreted from male mice induced lower Fos-immunoreactivity in the periglomerular (PG) cells of the AOBs in alpha1B-deficient female mice than in those of wild mice. The density of Fos-immunoreactive (Fos-ir) cells after exposure to female urine samples at the periglomerular cell layer of alpha1B-deficient male mice was lower than that of wild mice. Exposure of the VNO of beta3-deficient female mice to male urine samples also induced low Fos-ir cells in the periglomerular cell layer of the AOB. These data suggest the importance of the alpha1B and beta3 subunits of the N-type voltage-dependent Ca2+ channel for the pheromone signal transduction system"
Keywords:		"Animals Calcium Channels/*genetics Calcium Channels, N-Type/*genetics Electrophysiology Female Immunohistochemistry Male Mice Mice, Knockout Olfactory Bulb/*drug effects Pheromones/*pharmacology Proto-Oncogene Proteins c-fos/urine RNA/biosynthesis/genetic;"
Notes:		"MedlineMurakami, Manabu Matsui, Hitosi Shiraiwa, Takeshi Suzuki, Takashi Sasano, Hironobu Takahashi, Eiki Kashiwayanagi, Makoto eng Research Support, Non-U.S. Gov't Japan 2006/03/02 Biol Pharm Bull. 2006 Mar; 29(3):437-42. doi: 10.1248/bpb.29.437"