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Chemosphere

Title:		Effects of ionic strength on the production of short chain volatile hydrocarbons by Dunaliella salina (Teodoresco)
Author(s):		Munoz J; Mudge SM; Sandoval A;
Address:		"Departamento de Biotecnologia, Facultad de Ciencias, Universidad Catolica de la Santisima Concepcion, Concepcion, Chile"
Journal Title:		Chemosphere
Year:		2004
Volume:		54
Issue:		8
Page Number:		1267 - 1271
DOI:		10.1016/j.chemosphere.2003.09.019
ISSN/ISBN:		0045-6535 (Print) 0045-6535 (Linking)
Abstract:		"The effect of ionic strength on the production of short chain volatile hydrocarbons was studied in cultures of Dunaliella salina. Axenic cultures of D. salina were grown at three different ionic strengths 0.5, 2 and 3 M of NaCl in Johnson (J/1) culture medium [Journal of Bacteriology 95 (1968) 1461] under the following laboratory growth conditions: a 12:12 h photoperiod, 300 micromolm(-2)s(-1) of photosynthetic active radiation (PAR) provided by a fluorescent lamp of 40 W combined with a 100 W incandescent lamp at 20 +/- 1 degrees C at pH 7.5. C1 to C5 hydrocarbons were detected using a head space technique and GC-FID. Cell numbers and growth rate was greatest at 2 M NaCl 4.3 x 10(6) cellml(-1) after a 15 days period of culture. Maximum hydrocarbon production was measured in the concentration of 0.5 NaCl with lower production rates in the more concentrated solutions. The principal hydrocarbon was pentane at 0.5 M but was ethane in 2 and 3 M solutions. Production rates for individual compounds ranged between 0.13 and 22 x 10(-15) microgCcell(-1)h(-1). It is suggested that the ability to produce and release volatile organic compounds of D. salina is related to osmotic conditions established by the ionic strength of growth solution"
Keywords:		"Alkanes/chemistry/*metabolism Cell Count Chromatography, Gas/methods/statistics & numerical data Eukaryota/*growth & development/*metabolism Osmolar Concentration Sodium Chloride/pharmacology Time Factors Volatilization;"
Notes:		"MedlineMunoz, J Mudge, S M Sandoval, A eng Research Support, Non-U.S. Gov't England 2003/12/11 Chemosphere. 2004 Feb; 54(8):1267-71. doi: 10.1016/j.chemosphere.2003.09.019"