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J Food Prot

Title:		Antimicrobial activity of sub- and supercritical CO2 extracts of the green alga Dunaliella salina
Author(s):		Mendiola JA; Santoyo S; Cifuentes A; Reglero G; Ibanez E; Senorans FJ;
Address:		"Seccion Departamental de Ciencias de la Alimentacion, Universidad Autonoma de Madrid, Campus de Cantoblanco, 28049 Madrid, Spain"
Journal Title:		J Food Prot
Year:		2008
Volume:		71
Issue:		10
Page Number:		2138 - 2143
DOI:		10.4315/0362-028x-71.10.2138
ISSN/ISBN:		0362-028X (Print) 0362-028X (Linking)
Abstract:		"The objective of this research was to evaluate the antimicrobial activity of carbon dioxide extracts of the unicellular biflagellated green alga Dunaliella salina against Escherichia coli, Staphylococcus aureus, Candida albicans, and Aspergillus niger. The effects of different extraction pressures ranging from 185 to 442 bar and extraction temperatures ranging from 9.8 to 45.2 degrees C on the extracts' composition and consequently on their antimicrobial activities were investigated. The extracts were analyzed by gas chromatography-mass spectrometry in order to identify the compounds responsible for the antimicrobial activity detected. Fourteen different volatile compounds and several fatty acids were identified. The highest antimicrobial activity was obtained using 314 bar and 9.8 degrees C. Under these conditions, the presence of an indolic derivative that had never been reported in D. salina was detected in the extract, together with polyunsaturated fatty acids and compounds related to carotene metabolism, such as beta-ionone and neophytadiene, with known antimicrobial activity"
Keywords:		"Anti-Bacterial Agents/analysis/*pharmacology Aspergillus niger/drug effects Candida albicans/drug effects Carbon Dioxide/*pharmacology Chlorophyta/*chemistry Colony Count, Microbial Dose-Response Relationship, Drug Escherichia coli/drug effects Food Prese;"
Notes:		"MedlineMendiola, Jose A Santoyo, Susana Cifuentes, Alejandro Reglero, Guillermo Ibanez, Elena Senorans, F Javier eng Research Support, Non-U.S. Gov't 2008/10/23 J Food Prot. 2008 Oct; 71(10):2138-43. doi: 10.4315/0362-028x-71.10.2138"