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Lett Appl Microbiol

Title:		High-level gene expression in Lactobacillus plantarum using a pheromone-regulated bacteriocin promoter
Author(s):		Mathiesen G; Sorvig E; Blatny J; Naterstad K; Axelsson L; Eijsink VG;
Address:		"Department of Chemistry, Biotechnology and Food Science, Agricultural University of Norway, As, Norway"
Journal Title:		Lett Appl Microbiol
Year:		2004
Volume:		39
Issue:		2
Page Number:		137 - 143
DOI:		10.1111/j.1472-765X.2004.01551.x
ISSN/ISBN:		0266-8254 (Print) 0266-8254 (Linking)
Abstract:		"AIMS: To use promoters and regulatory genes involved in the production of the bacteriocin sakacin P to obtain high-level regulated gene expression in Lactobacillus plantarum. METHODS AND RESULTS: In a plasmid containing all three operons naturally involved in sakacin P production, the genes encoding sakacin P and its immunity protein were replaced by the aminopeptidase N gene from Lactococcus lactis (pepN) or the beta-glucuronidase gene from Escherichia coli (gusA). The new genes were precisely fused to the start codon of the sakacin P gene and the stop codon of the immunity gene. This set-up permitted regulated (external pheromone controlled) overexpression of both reporter genes in L. plantarum NC8. For PepN, production levels amounted to as much as 40% of total cellular protein. CONCLUSIONS: Promoters and regulatory genes involved in production of sakacin P are suitable for establishing inducible high-level gene expression in L. plantarum. SIGNIFICANCE AND IMPACT OF THE STUDY: This study describes a system for controllable gene expression in lactobacilli, giving some of the highest expression levels reported so far in this genus"
Keywords:		"Bacteriocin Plasmids/genetics/metabolism Bacteriocins/*genetics CD13 Antigens/analysis/biosynthesis/genetics Cloning, Molecular Escherichia coli/enzymology/genetics *Gene Expression Regulation, Bacterial Glucuronidase/analysis/biosynthesis/genetics Lactob;"
Notes:		"MedlineMathiesen, G Sorvig, E Blatny, J Naterstad, K Axelsson, L Eijsink, V G H eng Research Support, Non-U.S. Gov't England 2004/07/10 Lett Appl Microbiol. 2004; 39(2):137-43. doi: 10.1111/j.1472-765X.2004.01551.x"