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Neurosci Lett

Title:		Isolation of putative olfactory receptor sequences from pig nasal epithelium
Author(s):		Matarazzo V; Tirard A; Renucci M; Belaich A; Clement JL;
Address:		"UPR 9024, Laboratoire de Neurobiologie, CNRS, Marseille, France"
Journal Title:		Neurosci Lett
Year:		1998
Volume:		249
Issue:		2-Mar
Page Number:		87 - 90
DOI:		10.1016/s0304-3940(98)00375-9
ISSN/ISBN:		0304-3940 (Print) 0304-3940 (Linking)
Abstract:		"Binding to olfactory receptors is the first step in odorant and pheromonal recognition and discrimination. These receptors constitute one of the most important, although poorly known, families of neuronal receptors. In this study we used degenerated oligonucleotides and a RT-PCR approach to selectively amplify olfactory receptors in the nasal epithelium of the domestic pig Sus scrofa. Several combinations of oligonucleotide were tested and allowed the isolation of eleven different partial sequences belonging to the seven transmembrane olfactory receptor family. These receptors formed a separate family within the seven transmembrane receptor superfamily in pigs. Using the criteria of Ben Arie et al. [Ben-Arie N., Lancet D., Taylor C., Khen M., Walker N., Ledbetter DH., Carrozzo R., Patel K., Sheer D., Lehrah H. and North M., Hum. Mol. Genet., 3 (1994) 229-235], the 11 receptors described here can be classified into three known families and seven subfamilies (one known and six new)"
Keywords:		"Amino Acid Sequence Animals Humans Molecular Sequence Data Olfactory Mucosa/*chemistry Olfactory Receptor Neurons/*chemistry Oligonucleotides Phylogeny Polymerase Chain Reaction Sequence Homology, Amino Acid Swine/*genetics;"
Notes:		"MedlineMatarazzo, V Tirard, A Renucci, M Belaich, A Clement, J L eng Ireland 1998/07/31 Neurosci Lett. 1998 Jun 19; 249(2-3):87-90. doi: 10.1016/s0304-3940(98)00375-9"