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Appl Environ Microbiol

Title:		Development of bacteriocinogenic strains of Saccharomyces cerevisiae heterologously expressing and secreting the leaderless enterocin L50 peptides L50A and L50B from Enterococcus faecium L50
Author(s):		Basanta A; Herranz C; Gutierrez J; Criado R; Hernandez PE; Cintas LM;
Address:		"Departamento de Nutricion, Bromatologia y Tecnologia de los Alimentos, Facultad de Veterinaria, Universidad Complutense de Madrid, 28040 Madrid, Spain"
Journal Title:		Appl Environ Microbiol
Year:		2009
Volume:		20090213
Issue:		8
Page Number:		2382 - 2392
DOI:		10.1128/AEM.01476-08
ISSN/ISBN:		1098-5336 (Electronic) 0099-2240 (Print) 0099-2240 (Linking)
Abstract:		"A segregationally stable expression and secretion vector for Saccharomyces cerevisiae, named pYABD01, was constructed by cloning the yeast gene region encoding the mating pheromone alpha-factor 1 secretion signal (MFalpha1(s)) into the S. cerevisiae high-copy-number expression vector pYES2. The structural genes of the two leaderless peptides of enterocin L50 (EntL50A and EntL50B) from Enterococcus faecium L50 were cloned, separately (entL50A or entL50B) and together (entL50AB), into pYABD01 under the control of the galactose-inducible promoter P(GAL1). The generation of recombinant S. cerevisiae strains heterologously expressing and secreting biologically active EntL50A and EntL50B demonstrates the suitability of the MFalpha1(s)-containing vector pYABD01 to direct processing and secretion of these antimicrobial peptides through the S. cerevisiae Sec system"
Keywords:		"Anti-Infective Agents/*metabolism/pharmacology Bacteriocins/*biosynthesis/genetics/pharmacology Enterococcus faecium/*genetics Mass Spectrometry Microbial Sensitivity Tests Plasmids Promoter Regions, Genetic Protein Sorting Signals Recombinant Proteins/*b;"
Notes:		"MedlineBasanta, Antonio Herranz, Carmen Gutierrez, Jorge Criado, Raquel Hernandez, Pablo E Cintas, Luis M eng Research Support, Non-U.S. Gov't 2009/02/17 Appl Environ Microbiol. 2009 Apr; 75(8):2382-92. doi: 10.1128/AEM.01476-08. Epub 2009 Feb 13"