| Title: | Isothermal titration calorimetry - a new method for the quantification of microbial degradation of trace pollutants |
| Author(s): | Mariana F; Buchholz F; Harms H; Yong Z; Yao J; Maskow T; |
| Address: | "UFZ, Helmholtz Centre for Environmental Research, Dept. Environmental Microbiology, Permoserstr. 15, 04318 Leipzig, Germany" |
| DOI: | 10.1016/j.mimet.2010.03.024 |
| ISSN/ISBN: | 1872-8359 (Electronic) 0167-7012 (Linking) |
| Abstract: | "The environmental fate and, in particular, biodegradation rates of hydrophobic organic compounds (HOC) are of high interest due to the ubiquity, persistence, and potential health effects of these compounds. HOC tend to interact with bioreactor materials and sampling devices and are frequently volatile, so that conventionally derived degradation parameters are often biased. We report on the development and validation of a novel calorimetric approach that serves to gain real time information on the kinetics and the physiology of HOC bioconversion in aqueous systems while overcoming weaknesses of conventional biodegradation experiments. Soil bacteria Mycobacterium frederiksbergense LB501T, Rhodococcus erythropolis K2-3 and Pseudomonas putida G7 were exposed to pulsed titrations of dissolved anthracene, 4-(2,4-dichlorophenoxy)butyric acid or naphthalene, respectively, and the thermal responses were monitored. The combinations of strains and pollutants were selected as examples for complete and partial biodegradation and complete degradation with storage product formation, respectively. Heat production signals were interpreted thermodynamically and in terms of Michaelis-Menten kinetics. The half-saturation constant k(D) and the degradation rate r(D)(Max) were derived. Comparison with conventional methods shows the suitability to extract kinetic degradation parameters of organic trace pollutants from simple ITC experiments, while thermodynamic interpretation provided further information about the metabolic fate of HOC compounds" |
| Keywords: | "2, 4-Dichlorophenoxyacetic Acid/analogs & derivatives/chemistry/metabolism Anthracenes/chemistry/metabolism Bacteria/*chemistry/isolation & purification/*metabolism Biodegradation, Environmental Calorimetry/*methods Kinetics Naphthalenes/chemistry/metaboli;" |
| Notes: | "MedlineMariana, F Buchholz, F Harms, H Yong, Z Yao, J Maskow, T eng Evaluation Study Research Support, Non-U.S. Gov't Netherlands 2010/04/14 J Microbiol Methods. 2010 Jul; 82(1):42-8. doi: 10.1016/j.mimet.2010.03.024. Epub 2010 Apr 10" |
