| Title: | Degradation of a-factor by a Saccharomyces cerevisiae alpha-mating-type-specific endopeptidase: evidence for a role in recovery of cells from G1 arrest |
| Author(s): | Marcus S; Xue CB; Naider F; Becker JM; |
| Address: | "Department of Microbiology, University of Tennessee, Knoxville 37996" |
| DOI: | 10.1128/mcb.11.2.1030-1039.1991 |
| ISSN/ISBN: | 0270-7306 (Print) 1098-5549 (Electronic) 0270-7306 (Linking) |
| Abstract: | "Mating response between opposite mating types of Saccharomyces cerevisiae is dependent upon alpha factor, a tridecapeptide, and a-factor, an isoprenylated, methyl esterified dodecapeptide whose interaction with the alpha target cell has not been characterized. We report on the first biochemical and physiological evidence of an alpha-mating-type-specific a-factor-degrading activity. Radioiodinated a-factor was used to identify the a-factor-degrading activity, which is cell associated, endoproteolytic, and not required for response to pheromone. a-factor degradation was not energy dependent, nor did it require pheromone internalization or interaction with its receptor. Phenylmethylsulfonyl fluoride and tosyl-L-arginyl-methyl ester inhibited degradation of a-factor and increased the time required by alpha cells to recover from a-factor-induced growth arrest and morphological alteration, providing evidence that a-factor degradation plays a role in the recovery of alpha cells from the pheromone response" |
| Keywords: | Amino Acid Sequence Endopeptidases/*metabolism G1 Phase Kinetics Mating Factor Molecular Sequence Data Peptides/isolation & purification/*metabolism Pheromones/metabolism Saccharomyces cerevisiae/cytology/genetics/*metabolism Structure-Activity Relationsh; |
| Notes: | "MedlineMarcus, S Xue, C B Naider, F Becker, J M eng GM-22086/GM/NIGMS NIH HHS/ GM-22087/GM/NIGMS NIH HHS/ Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. 1991/02/01 Mol Cell Biol. 1991 Feb; 11(2):1030-9. doi: 10.1128/mcb.11.2.1030-1039.1991" |
