| Title: | Insights on pH-dependent conformational changes of mosquito odorant binding proteins by molecular dynamics simulations |
| Author(s): | Manoharan M; Fuchs PF; Sowdhamini R; Offmann B; |
| Address: | "a DSIMB , University of La Reunion , 97400 , Saint-Denis, La Reunion , France" |
| DOI: | 10.1080/07391102.2013.834118 |
| ISSN/ISBN: | 1538-0254 (Electronic) 0739-1102 (Linking) |
| Abstract: | "Chemical recognition plays an important role for the survival and reproduction of many insect species. Odorant binding proteins (OBPs) are the primary components of the insect olfactory mechanism and have been documented to play an important role in the host-seeking mechanism of mosquitoes. They are 'transport proteins' believed to transport odorant molecules from the external environment to their respective membrane targets, the olfactory receptors. The mechanism by which this transport occurs in mosquitoes remains a conundrum in this field. Nevertheless, OBPs have proved to be amenable to conformational changes mediated by a pH change in other insect species. In this paper, the effect of pH on the conformational flexibility of mosquito OBPs is assessed computationally using molecular dynamics simulations of a mosquito OBP 'CquiOBP1' bound to its pheromone 3OG (PDB ID: 3OGN). Conformational twist of a loop, driven by a set of well-characterized changes in intramolecular interactions of the loop, is demonstrated. The concomitant (i) closure of what is believed to be the entrance of the binding pocket, (ii) expansion of what could be an exit site, and (iii) migration of the ligand towards this putative exit site provide preliminary insights into the mechanism of ligand binding and release of these proteins in mosquitoes. The correlation of our results with previous experimental observations based on NMR studies help us provide a cardinal illustration on one of the probable dynamics and mechanism by which certain mosquito OBPs could deliver their ligand to their membrane-bound receptors at specific pH conditions" |
| Keywords: | "Animals Binding Sites Culicidae Hydrogen-Ion Concentration Insect Proteins/*chemistry *Molecular Dynamics Simulation Pheromones/chemistry Protein Conformation Receptors, Odorant/*chemistry dynamics ligand binding mosquito odorant binding protein pH;" |
| Notes: | "MedlineManoharan, Malini Fuchs, Patrick F J Sowdhamini, Ramanathan Offmann, Bernard eng Research Support, Non-U.S. Gov't England 2013/09/14 J Biomol Struct Dyn. 2014; 32(11):1742-51. doi: 10.1080/07391102.2013.834118. Epub 2013 Sep 13" |
