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J Chromatogr A

Title:		An anion-exchange high-performance liquid chromatography method coupled to total organic carbon determination for the analysis of water-soluble organic aerosols
Author(s):		Mancinelli V; Rinaldi M; Finessi E; Emblico L; Mircea M; Fuzzi S; Facchini MC; Decesari S;
Address:		"Institute of Atmospheric Sciences and Climate, National Research Council, via Gobetti 101, 40129 Bologna, Italy"
Journal Title:		J Chromatogr A
Year:		2007
Volume:		20070316
Issue:		2
Page Number:		385 - 389
DOI:		10.1016/j.chroma.2007.02.111
ISSN/ISBN:		0021-9673 (Print) 0021-9673 (Linking)
Abstract:		"The chemical composition of water-soluble organic carbon (WSOC) in atmospheric aerosol particles is largely unexplored, due to the myriad of individual compounds, which has hampered attempts to attain a full characterization at the molecular level. An alternative approach, focusing on the analysis of a few main chemical classes, allowed the quantitative fractionation of WSOC into neutral compounds (NC), mono- and di-acids (MDA) and polyacids (PA) through an anion-exchange liquid chromatographic method. Previous attempts to quantify NC, MDA and PA relied on a low-pressure chromatographic technique using a volatile buffer, followed by total organic carbon (TOC) analysis of the fractions, or alternatively on a faster HPLC-UV method which provided a quantification of the fractions based on empirical relationships between UV signal and TOC concentration. Here, we report an upgraded anion-exchange HPLC technique, allowing direct TOC analysis of the eluted fractions, without any pre-treatment, thus permitting a great simplification of quantitative analysis and preventing sample losses. The new HPLC-TOC methodology, employing completely inorganic eluents shows the same efficiency of the former HPLC-UV method employing organic additives, with the exception of phenolic compounds, which are retained on the column by secondary interactions, and low-molecular weight tricarboxylic acids, which are recovered among MDA. The new anion-exchange liquid chromatographic method can recover a substantial amount (86-100%) of water-soluble organic carbon from atmospheric aerosol extracts, thus allowing a higher retention and fractionation capacity with respect to alternative techniques, like solid phase extraction"
Keywords:		"*Aerosols *Anion Exchange Resins Carbon/*analysis Chromatography, High Pressure Liquid/*methods Chromatography, Ion Exchange/*methods Organic Chemicals/*analysis Reference Standards Solubility Spectrophotometry, Ultraviolet Water;"
Notes:		"MedlineMancinelli, Valeriana Rinaldi, Matteo Finessi, Emanuela Emblico, Lorenza Mircea, Mihaela Fuzzi, Sandro Facchini, Maria Cristina Decesari, Stefano eng Research Support, Non-U.S. Gov't Netherlands 2007/04/07 J Chromatogr A. 2007 May 18; 1149(2):385-9. doi: 10.1016/j.chroma.2007.02.111. Epub 2007 Mar 16"