Bedoukian   RussellIPM   RussellIPM   Piezoelectric Micro-Sprayer


Home
Animal Taxa
Plant Taxa
Semiochemicals
Floral Compounds
Semiochemical Detail
Semiochemicals & Taxa
Synthesis
Control
Invasive spp.
References

Abstract

Guide

Alphascents
Pherobio
InsectScience
E-Econex
Counterpart-Semiochemicals
Print
Email to a Friend
Kindly Donate for The Pherobase

« Previous AbstractAn efficient Escherichia coli expression system for the production of a functional N-terminal domain of the T1R3 taste receptor    Next AbstractContrasting responses of silver birch VOC emissions to short- and long-term herbivory »

Pestic Biochem Physiol


Title:"Expression profile of the entire detoxification gene inventory of the western honeybee, Apis mellifera across life stages"
Author(s):Maiwald F; Haas J; Hertlein G; Lueke B; Roesner J; Nauen R;
Address:"Bayer AG, Crop Science Division, R&D, Pest Control, 40789 Monheim am Rhein, Germany. Bayer AG, Crop Science Division, R&D, Pest Control, 40789 Monheim am Rhein, Germany. Electronic address: ralf.nauen@bayer.com"
Journal Title:Pestic Biochem Physiol
Year:2023
Volume:20230328
Issue:
Page Number:105410 -
DOI: 10.1016/j.pestbp.2023.105410
ISSN/ISBN:1095-9939 (Electronic) 0048-3575 (Linking)
Abstract:"The western honeybee, Apis mellifera, is a managed pollinator of many crops and potentially exposed to a wide range of foreign compounds, including pesticides throughout its life cycle. Honeybees as well as other insects recruit molecular defense mechanisms to facilitate the detoxification of xenobiotic compounds. The inventory of detoxification genes (DETOXome) is comprised of five protein superfamilies: cytochrome P450 monooxygenases (P450), carboxylesterases, glutathione S-transferases (GST), UDP-glycosyl transferases (UGT) and ATP-binding cassette (ABC) transporters. Here we characterized the gene expression profile of the entire honeybee DETOXome by analyzing 47 transcriptomes across the honeybee life cycle, including different larval instars, pupae, and adults. All life stages were well separated by principal component analysis, and K-means clustering revealed distinct temporal patterns of gene expression. Indeed, >50% of the honeybee detoxification gene inventory is found in one cluster and follows strikingly similar expression profiles, i.e., increased expression during larval development, followed by a sharp decline after pupation and a steep increase again in adults. This cluster includes 29 P450 genes dominated by CYP3 and CYP4 clan members, 15 ABC transporter genes mostly belonging to the ABCC subfamily and 13 carboxylesterase genes including almost all members involved in dietary/detox and hormone/semiochemical processing. RT-qPCR analysis of selected detoxification genes from all families revealed high expression levels in various tissues, especially Malpighian tubules, fatbody and midgut, supporting the view that these tissues are essential for metabolic clearance of environmental toxins and pollutants in honeybees. Our study is meant to spark further research on the molecular basis of detoxification in this critical pollinator to better understand and evaluate negative impacts from potentially toxic substances. Additionally, the entire gene set of 47 transcriptomes collected and analyzed provides a valuable resource for future honeybee research across different disciplines"
Keywords:Bees/genetics Animals *Pesticides Insecta Cytochrome P-450 Enzyme System/genetics/metabolism Carboxylic Ester Hydrolases Transferases Cytochrome P450 Detoxification Honey bee Metabolism Pesticides Pollinators;
Notes:"MedlineMaiwald, Frank Haas, Julian Hertlein, Gillian Lueke, Bettina Roesner, Janin Nauen, Ralf eng 2023/04/28 Pestic Biochem Physiol. 2023 May; 192:105410. doi: 10.1016/j.pestbp.2023.105410. Epub 2023 Mar 28"

 
Back to top
 
Citation: El-Sayed AM 2024. The Pherobase: Database of Pheromones and Semiochemicals. <http://www.pherobase.com>.
© 2003-2024 The Pherobase - Extensive Database of Pheromones and Semiochemicals. Ashraf M. El-Sayed.
Page created on 22-11-2024