« Previous Abstract"Formulation of goat's milk yogurt with fig powder: Aromatic profile, physicochemical and microbiological characteristics"    Next AbstractIdentifying aroma-active compounds in coffee-flavored dairy beverages »

Microorganisms

Title:		"Klebsiella pneumoniae Volatile Organic Compounds (VOCs) Protect Artemia salina from Fish Pathogen Aeromonas sp.: A Combined In Vitro, In Vivo, and In Silico Approach"
Author(s):		Mahmud ML; Islam S; Biswas S; Mortuza MG; Paul GK; Uddin MS; Akhtar EE; Saleh MA; Zaman S; Syed A; Elgorban AM; Zaghloul NSS;
Address:		"Microbiology Laboratory, Department of Genetic Engineering and Biotechnology, University of Rajshahi, Rajshahi 6205, Bangladesh. Department of Science and Humanities, Bangladesh Army International University of Science and Technology, Cumilla 3500, Bangladesh. Bangladesh Reference Institute for Chemical Measurements (BRICM), Bangladesh Council of Scientific and Industrial Research (BCSIR), Dhaka 1205, Bangladesh. Department of Botany and Microbiology, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia. Bristol Centre for Functional Nanomaterials, HH Wills Physics Laboratory, Tyndall Avenue, University of Bristol, Bristol BS8 1FD, UK"
Journal Title:		Microorganisms
Year:		2023
Volume:		20230110
Issue:		1
Page Number:		-
DOI:		10.3390/microorganisms11010172
ISSN/ISBN:		2076-2607 (Print) 2076-2607 (Electronic) 2076-2607 (Linking)
Abstract:		"Antibiotic resistance is an alarming threat all over the world, and the biofilm formation efficacy of bacteria is making the situation worse. The antagonistic efficacy of Klebsiella pneumoniae against one of the known fish pathogens, Aeromonas sp., is examined in this study. Moreover, Aeromonas sp.'s biofilm formation ability and in vivo pathogenicity on Artemia salina are also justified here. Firstly, six selected bacterial strains were used to obtain antimicrobial compounds against this pathogenic strain. Among those, Klebsiella pneumoniae, another pathogenic bacterium, surprisingly demonstrated remarkable antagonistic activity against Aeromonas sp. in both in vitro and in vivo assays. The biofilm distrusting potentiality of Klebsiella pneumoniae's cell-free supernatants (CFSs) was likewise found to be around 56%. Furthermore, the volatile compounds of Klebsiella pneumoniae were identified by GC-MS in order to explore compounds with antibacterial efficacy against Aeromonas sp. through an in silico study, where 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase (MTAN) (PDB: 5B7P) was chosen as a target protein for its unique characteristics and pathogenicity. Several volatile compounds, such as oxime- methoxy-phenyl-, fluoren-9-ol, 3,6-dimethoxy-9-(2-phenylethynyl)-, and 2H-indol-2-one, 1,3-dihydro- showed a strong binding affinity, with free energy of -6.7, -7.1, and -6.4 Kcal/mol, respectively, in complexes with the protein MTAN. Moreover, the root-mean-square deviation, solvent-accessible surface area, radius of gyration, root-mean-square fluctuations, and hydrogen bonds were used to ensure the binding stability of the docked complexes in the atomistic simulation. Thus, Klebsiella pneumoniae and its potential compounds can be employed as an alternative to antibiotics for aquaculture, demonstrating their effectiveness in suppressing Aeromonas sp"
Keywords:		Aeromonas sp.Artemia salina Klebsiella pneumoniae antibiotics pathogenic infection protection;
Notes:		"PubMed-not-MEDLINEMahmud, Md Liton Islam, Shirmin Biswas, Suvro Mortuza, Md Golam Paul, Gobindo Kumar Uddin, Md Salah Akhtar-E-Ekram, Md Saleh, Md Abu Zaman, Shahriar Syed, Asad Elgorban, Abdallah M Zaghloul, Nouf S S eng Switzerland 2023/01/22 Microorganisms. 2023 Jan 10; 11(1):172. doi: 10.3390/microorganisms11010172"