| Title: | Biochemical and genetic characterization of a competence pheromone from B. subtilis |
| Author(s): | Magnuson R; Solomon J; Grossman AD; |
| Address: | "Department of Biology, Massachusetts Institute of Technology, Cambridge 02139" |
| DOI: | 10.1016/0092-8674(94)90313-1 |
| ISSN/ISBN: | 0092-8674 (Print) 0092-8674 (Linking) |
| Abstract: | "We have purified and characterized a modified peptide pheromone that accumulates in culture medium as B. subtilis grows to high density. This pheromone is required for the development of genetic competence. When added to cells at low density, the pheromone induces the premature development of competence. The peptide moiety of the pheromone matches nine of the last ten amino acids predicted from a 55 codon open reading frame, comX. comX and comQ, the gene immediately upstream of comX, are required for production of the pheromone. Response to the pheromone requires the comP-comA two-component regulatory system and the oligopeptide permease encoded by spo0K. Spo0K could transport the pheromone into the cell, or function as a receptor, binding the pheromone and sending a transmembrane signal, leading to activation of the ComA transcription factor and induction of competence development" |
| Keywords: | "Amino Acid Sequence Bacillus subtilis/*chemistry/genetics/growth & development Bacterial Proteins/chemistry/*genetics/isolation & purification Base Sequence Cloning, Molecular Gene Expression Regulation, Bacterial/genetics Genes, Bacterial/*genetics Genes;" |
| Notes: | "MedlineMagnuson, R Solomon, J Grossman, A D eng GM41934/GM/NIGMS NIH HHS/ RR00317/RR/NCRR NIH HHS/ Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. 1994/04/22 Cell. 1994 Apr 22; 77(2):207-16. doi: 10.1016/0092-8674(94)90313-1" |
