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Chemosphere

Title:		CYP4CJ1-mediated gossypol and tannic acid tolerance in Aphis gossypii Glover
Author(s):		Ma K; Li F; Tang Q; Liang P; Liu Y; Zhang B; Gao X;
Address:		"Department of Entomology, China Agricultural University, Beijing, 100193, China. College of Resources and Environment, Henan Institute of Science and Technology, Xinxiang, 453000, China. Department of Entomology, China Agricultural University, Beijing, 100193, China. Electronic address: gaoxiwu@263.net.cn"
Journal Title:		Chemosphere
Year:		2019
Volume:		20181204
Issue:
Page Number:		961 - 970
DOI:		10.1016/j.chemosphere.2018.12.025
ISSN/ISBN:		1879-1298 (Electronic) 0045-6535 (Linking)
Abstract:		"Cytochrome P450 monooxygenases play a key role in herbivorous pest adaptation to host plants by the detoxification against plant allelochemicals. A new P450 gene (CYP4CJ1) was identified from Aphis gossypii, which displayed a positive response to plant allelochemicals. The transcript levels of CYP4CJ1 could be significantly induced by both gossypol and tannic acid. Knockdown of CYP4CJ1 increased the sensitivity of A. gossypii to these two plant allelochemicals. These results suggest that CYP4CJ1 could be involved in the tolerance of A. gossypii to some plant allelochemicals. Subsequently, we examined the regulatory mechanism of CYP4CJ1 based on the transcriptional and post-transcriptional level. A promoter region from -1422 to -1166 of CYP4CJ1 was identified, which was an essential plant allelochemical responsive region. In addition, miR-4133-3p was found to participate in the regulation of CYP4CJ1 post-transcriptionally. Our results suggest that the transcript abundance of CYP4CJ1, following the exposure of A. gossypii to gossypol and tannic acid can be attributed to both the transcriptional and post-transcriptional regulation mechanisms. These results are important for understanding the roles of P450s in the plant allelochemical tolerance of A. gossypii"
Keywords:		Animals Cytochrome P-450 Enzyme System/*metabolism Gossypol/*chemistry Tannins/*chemistry Aphis gossypii Cytochrome P450 Gossypol Regulatory mechanism Tannic acid;
Notes:		"MedlineMa, Kangsheng Li, Fen Tang, Qiuling Liang, Pingzhuo Liu, Ying Zhang, Baizhong Gao, Xiwu eng England 2018/12/21 Chemosphere. 2019 Mar; 219:961-970. doi: 10.1016/j.chemosphere.2018.12.025. Epub 2018 Dec 4"