| Title: | A new role for MCM1 in yeast: cell cycle regulation of SW15 transcription |
| Author(s): | Lydall D; Ammerer G; Nasmyth K; |
| Address: | "Research Institute of Molecular Pathology (IMP), University of Vienna, Austria" |
| ISSN/ISBN: | 0890-9369 (Print) 0890-9369 (Linking) |
| Abstract: | "In the yeast Saccharomyces cerevisiae cell cycle-regulated SW15 transcription is essential for ensuring that mother and not daughter cells switch mating type. We have identified a 55-bp promoter sequence that appears to be responsible for restricting transcription to the late S, G2, and M phases of the cell cycle. Two proteins, MCM1, a transcription factor described previously, and SFF (SWI five factor, a newly identified factor) bind this sequence in vitro. MCM1 binds the DNA tightly on its own, but SFF will only bind as part of a ternary complex with MCM1. We observe a strong correlation between the ability of mutated SWI5 promoter sequences to form a ternary MCM1-SFF-containing complex in vitro and to activate transcription in vivo, which suggests that efficient transcription requires that both proteins bind DNA. Through its interactions with cell type-specific coactivators and corepressors, MCM1 controls cell type-specific expression of pheromone and receptor genes. By analogy, we propose that SFF enables MCM1 to function as a part of a cell cycle-regulated transcription complex" |
| Keywords: | "Base Composition Base Sequence *Cell Cycle Fungal Proteins/chemistry/genetics/*metabolism *Gene Expression Regulation, Fungal Minichromosome Maintenance 1 Protein Molecular Sequence Data Promoter Regions, Genetic Protein Binding RNA, Fungal/metabolism Sac;" |
| Notes: | "MedlineLydall, D Ammerer, G Nasmyth, K eng Research Support, Non-U.S. Gov't 1991/12/01 Genes Dev. 1991 Dec; 5(12B):2405-19. doi: 10.1101/gad.5.12b.2405" |
