| Title: | Genome Sequencing of Pantoea agglomerans C1 Provides Insights into Molecular and Genetic Mechanisms of Plant Growth-Promotion and Tolerance to Heavy Metals |
| Author(s): | Luziatelli F; Ficca AG; Cardarelli M; Melini F; Cavalieri A; Ruzzi M; |
| Address: | "Department for Innovation in Biological, Agrofood and Forest systems (DIBAF), University of Tuscia, via C. de Lellis, snc, I-01100 Viterbo, Italy. CREA Research Centre for Vegetable and Ornamental Crops, I-84098 Pontecagnano, Italy. CREA Research Centre for Food and Nutrition, Via Ardeatina 546, I-00178 Rome, Italy. Department of Plant and Environmental Sciences, University of Copenhagen, DK-1871 Frederiksberg, Denmark" |
| DOI: | 10.3390/microorganisms8020153 |
| ISSN/ISBN: | 2076-2607 (Print) 2076-2607 (Electronic) 2076-2607 (Linking) |
| Abstract: | "Distinctive strains of Pantoea are used as soil inoculants for their ability to promote plant growth. Pantoea agglomerans strain C1, previously isolated from the phyllosphere of lettuce, can produce indole-3-acetic acid (IAA), solubilize phosphate, and inhibit plant pathogens, such as Erwinia amylovora. In this paper, the complete genome sequence of strain C1 is reported. In addition, experimental evidence is provided on how the strain tolerates arsenate As (V) up to 100 mM, and on how secreted metabolites like IAA and siderophores act as biostimulants in tomato cuttings. The strain has a circular chromosome and two prophages for a total genome of 4,846,925-bp, with a DNA G+C content of 55.2%. Genes related to plant growth promotion and biocontrol activity, such as those associated with IAA and spermidine synthesis, solubilization of inorganic phosphate, acquisition of ferrous iron, and production of volatile organic compounds, siderophores and GABA, were found in the genome of strain C1. Genome analysis also provided better understanding of the mechanisms underlying strain resistance to multiple toxic heavy metals and transmission of these genes by horizontal gene transfer. Findings suggested that strain C1 exhibits high biotechnological potential as plant growth-promoting bacterium in heavy metal polluted soils" |
| Keywords: | Pantoea agglomerans Solanum lycopersicum L.arsenic resistance complete genome horizontal gene transfer indole-3-acetic acid plant growth-promotion siderophores; |
| Notes: | "PubMed-not-MEDLINELuziatelli, Francesca Ficca, Anna Grazia Cardarelli, Mariateresa Melini, Francesca Cavalieri, Andrea Ruzzi, Maurizio eng Switzerland 2020/01/26 Microorganisms. 2020 Jan 22; 8(2):153. doi: 10.3390/microorganisms8020153" |
