| Title: | Herbivore-induced tomato plant volatiles lead to the reduction of insecticides susceptibility in Spodoptera litura |
| Author(s): | Luo YS; Abdellah YAY; Hafeez M; Yang X; Hou WT; Kong XH; Wang RL; |
| Address: | "Guangdong Provincial Key Laboratory of Eco-circular Agriculture, South China Agricultural University, Guangzhou 510642, China; Guangdong Engineering Technology Research Centre of Modern Eco-agriculture and Circular Agriculture, Guangzhou 510642, China; Key Laboratory of Agro-Environment in the Tropics, Ministry of Agriculture and Rural Affairs, South China Agricultural University, Guangzhou, 510642, China. State Key Laboratory of Rice Biology & Ministry of Agriculture Key Lab of Molecular Biology of Crop Pathogens and Insects, Institute of Insect Sciences, Zhejiang University, Hangzhou 310058, China. Guangdong Provincial Key Laboratory of Eco-circular Agriculture, South China Agricultural University, Guangzhou 510642, China; Guangdong Engineering Technology Research Centre of Modern Eco-agriculture and Circular Agriculture, Guangzhou 510642, China; Key Laboratory of Agro-Environment in the Tropics, Ministry of Agriculture and Rural Affairs, South China Agricultural University, Guangzhou, 510642, China. Electronic address: xuhuikong@scau.edu.cn. Guangdong Provincial Key Laboratory of Eco-circular Agriculture, South China Agricultural University, Guangzhou 510642, China; Guangdong Engineering Technology Research Centre of Modern Eco-agriculture and Circular Agriculture, Guangzhou 510642, China; Key Laboratory of Agro-Environment in the Tropics, Ministry of Agriculture and Rural Affairs, South China Agricultural University, Guangzhou, 510642, China. Electronic address: rlw2009@scau.edu.cn" |
| DOI: | 10.1016/j.pestbp.2022.105215 |
| ISSN/ISBN: | 1095-9939 (Electronic) 0048-3575 (Linking) |
| Abstract: | "Herbivore-induced plant volatiles (HIPVs) have been associated with plant-plant-herbivorous-natural enemies communication and an enhanced response to the subsequent attack. Spodoptera litura is a serious cosmopolitan pest that has developed a high level of resistance to many insecticides. However, the underlying molecular and biochemical mechanism by which HIPV priming reduces S. litura larval sensitivity to insecticides remains largely unknown. This study was conducted to explore the potential of volatile from undamaged, or artificially damaged, or S. litura-damaged tomato plants on the susceptibility of S. litura to the insecticides beta-cypermethrin indoxacarb and chlorpyrifos. We found that larvae exposed to volatile from S. litura-damaged or artificially damaged tomato plants were significantly less susceptible to the three insecticides than those exposed to volatile from undamaged tomato plants. Elevated activities of detoxifying enzymes [cytochrome P450 monooxygenases (P450s), glutathione S-transferases (GSTs), and esterases (ESTs)], were expressed in S. litura larvae exposed to volatile from S. litura-damaged tomato plants than those exposed to volatile from undamaged tomato plants. Similarly, seven detoxification-related genes [GSTs (SlGSTe1, SlGSTo1, and SlGSTe3) and P450s (CYP6B48, CYP9A40, CYP321A7, and CYP321B1)] in the midgut and fat body of larvae were up-regulated under exposure to volatile from S. litura-damaged tomato plants. Increased volatile organic compounds emissions were detected in the headspace of tomato plants damaged by S. litura compared to the undamaged plants. Collectively, these findings suggest that HIPVs can considerably reduce caterpillar susceptibility to insecticides, possibly through induction-enhanced detoxification mechanisms, and provide valuable information for implementing an effective integrated pest management strategy" |
| Keywords: | Animals *Chlorpyrifos/pharmacology Cytochrome P-450 Enzyme System/genetics Esterases Glutathione Herbivory *Insecticides/toxicity Larva *Solanum lycopersicum Spodoptera Transferases/pharmacology *Volatile Organic Compounds/pharmacology Herbivore-induced p; |
| Notes: | "MedlineLuo, Yu-Sen Abdellah, Yousif Abdelrahman Yousif Hafeez, Muhammad Yang, Xi Hou, Wen-Tao Kong, Xu-Hui Wang, Rui-Long eng 2022/09/21 Pestic Biochem Physiol. 2022 Oct; 187:105215. doi: 10.1016/j.pestbp.2022.105215. Epub 2022 Aug 25" |
