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Molecules

Title:		Lemon Oils Attenuate the Pathogenicity of Pseudomonas aeruginosa by Quorum Sensing Inhibition
Author(s):		Luciardi MC; Blazquez MA; Alberto MR; Cartagena E; Arena ME;
Address:		"INBIOFAL (Institute of Pharmaceutical and Food Biotechnology), CONICET NOA Sur, Avenida Kirchner 1900, Tucuman 4000, Argentina. Facultad de Bioquimica, Quimica y Farmacia, Universidad Nacional de Tucuman (UNT), Ayacucho 471, Tucuman 4000, Argentina. Departament de Farmacologia, Facultat de Farmacia, Universitat de Valencia, Avd. Vicent Andres Estelles s/n, 46100 Burjasot, Valencia, Spain"
Journal Title:		Molecules
Year:		2021
Volume:		20210512
Issue:		10
Page Number:		-
DOI:		10.3390/molecules26102863
ISSN/ISBN:		1420-3049 (Electronic) 1420-3049 (Linking)
Abstract:		"The chemical composition of three Citrus limon oils: lemon essential oil (LEO), lemon terpenes (LT) and lemon essence (LE), and their influence in the virulence factors production and motility (swarming and swimming) of two Pseudomonas aeruginosa strains (ATCC 27853 and a multidrug-resistant HT5) were investigated. The main compound, limonene, was also tested in biological assays. Eighty-four compounds, accounting for a relative peak area of 99.23%, 98.58% and 99.64%, were identified by GC/MS. Limonene (59-60%), gamma-terpinene (10-11%) and beta-pinene (7-15%) were the main compounds. All lemon oils inhibited specific biofilm production and bacterial metabolic activities into biofilm in a dose-dependent manner (20-65%, in the range of 0.1-4 mg mL(-1)) of both strains. Besides, all samples inhibited about 50% of the elastase activity at 0.1 mg mL(-1). Pyocyanin biosynthesis decreases until 64% (0.1-4 mg mL(-1)) for both strains. Swarming motility of P. aeruginosa ATCC 27853 was completely inhibited by 2 mg mL(-1) of lemon oils. Furthermore, a decrease (29-55%, 0.1-4 mg mL(-1)) in the synthesis of Quorum sensing (QS) signals was observed. The oils showed higher biological activities than limonene. Hence, their ability to control the biofilm of P. aeruginosa and reduce the production of virulence factors regulated by QS makes lemon oils good candidates to be applied as preservatives in the food processing industry"
Keywords:		"Anti-Bacterial Agents/chemistry/*pharmacology Bacterial Proteins/metabolism Bicyclic Monoterpenes/chemistry/pharmacology Biofilms/drug effects Citrus/*chemistry Cyclohexane Monoterpenes/chemistry/pharmacology Drug Resistance, Multiple, Bacterial/drug effe;"
Notes:		"MedlineLuciardi, Maria Constanza Blazquez, Maria Amparo Alberto, Maria Rosa Cartagena, Elena Arena, Mario Eduardo eng PIUNT D26/638-1/SCAIT-UNT/ PICT 02071 and 02514/ANPCyT/ PIP 662/CONICET/ Switzerland 2021/06/03 Molecules. 2021 May 12; 26(10):2863. doi: 10.3390/molecules26102863"