| Title: | Cloning and characterization of a coronatine-regulated tyrosine aminotransferase from Arabidopsis |
| Author(s): | Lopukhina A; Dettenberg M; Weiler EW; Hollander-Czytko H; |
| Address: | "Lehrstuhl fur Pflanzenphysiologie, Ruhr-Universitat, D-44780 Bochum, Germany" |
| ISSN/ISBN: | 0032-0889 (Print) 1532-2548 (Electronic) 0032-0889 (Linking) |
| Abstract: | "In plants, the phytotoxin coronatine, which is an analog of the octadecanoids 12-oxo-phytodienoic acid and/or jasmonic acid, gives rise to a number of physiological responses similar to those of octadecanoids. To further elucidate the physiological role of these compounds, the differential RNA display technique was used to isolate a number of novel octadecanoid-inducible genes expressed in coronatine-treated Arabidopsis. Among these, a cDNA clone was identified that was similar to known tyrosine aminotransferases (TATs). The function was verified with the expressed recombinant protein. In Arabidopsis, the protein is present as a multimer of 98 kD, with a monomer of an apparent molecular mass of 47 kD. TAT mRNA could be induced within 2 h by various octadecanoids and by wounding of the plants. Accumulation of the TAT protein and a 5- to 7-fold increase in its enzymatic activity was observed 7 to 9 h after application of octadecanoids, coronatine, or wounding. The potential role of TAT in the defense response to herbivores and pathogens is discussed" |
| Keywords: | "Amino Acid Sequence Amino Acids/*physiology Animals Arabidopsis/drug effects/enzymology/*genetics Cloning, Molecular DNA, Complementary DNA, Plant Gene Expression Profiling *Gene Expression Regulation, Enzymologic *Gene Expression Regulation, Plant Humans;" |
| Notes: | "MedlineLopukhina, A Dettenberg, M Weiler, E W Hollander-Czytko, H eng Research Support, Non-U.S. Gov't 2001/08/14 Plant Physiol. 2001 Aug; 126(4):1678-87. doi: 10.1104/pp.126.4.1678" |
