| Title: | "Cloning and functional characterization of three new pheromone receptors from the diamondback moth, Plutella xylostella" |
| Author(s): | Liu Y; Liu Y; Jiang X; Wang G; |
| Address: | "State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China; College of Life Science, Hunan Normal University, Changsha 410006, China. State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China. Electronic address: yangliu@ippcaas.cn. College of Plant Protection, Anhui Agricultural University, Hefei 230036, China. State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China. Electronic address: wangguirong@caas.cn" |
| DOI: | 10.1016/j.jinsphys.2018.02.005 |
| ISSN/ISBN: | 1879-1611 (Electronic) 0022-1910 (Linking) |
| Abstract: | "The highly specialized olfactory receptor neurons (ORNs) on the antennae of male moths can recognize blends of several pheromone components. In previous studies, a total of six candidate pheromone receptor (PR) genes were cloned and functionally characterized in the diamondback moth, Plutella xylostella. In the present work, we report on three novel candidate pheromone receptor genes: PxylOR8, PxylOR41, and PxylOR45 in the same species. Gene expression analysis revealed that PxylOR8 is specifically expressed in female adult antennae, while PxylOR41 and PxylOR45 are expressed in antennae in both sexes, but with a male bias. In situ hybridization revealed that PxylOR8, PxylOR41 and PxylOR45 are localized in long trichoid sensilla. Functional analyses on the three pheromone receptor genes were then performed using the heterologous expression system of Xenopus oocytes. PxylOR41 was tuned to two minor pheromone components Z9-14:Ac, Z9-14:OH, and their analog Z9-14:Ald. PxylOR8 and PxylOR45 did not respond to any tested pheromone components and analogs. These results may contribute to clarifying how pheromone detection works in P. xylostella" |
| Keywords: | "Amino Acid Sequence Animals Arthropod Antennae/*metabolism Cloning, Molecular Female Insect Proteins/chemistry/*genetics/metabolism Male Moths/physiology Phylogeny Receptors, Pheromone/chemistry/*genetics/metabolism Sensilla/metabolism Sequence Alignment;" |
| Notes: | "MedlineLiu, Yipeng Liu, Yang Jiang, Xingchuan Wang, Guirong eng Research Support, Non-U.S. Gov't England 2018/02/14 J Insect Physiol. 2018 May-Jun; 107:14-22. doi: 10.1016/j.jinsphys.2018.02.005. Epub 2018 Feb 10" |
