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Proc Natl Acad Sci U S A

Title:		Gene characterized for membrane desaturase that produces (E)-11 isomers of mono- and diunsaturated fatty acids
Author(s):		Liu W; Jiao H; Murray NC; O'Connor M; Roelofs WL;
Address:		"Department of Entomology, New York State Agricultural Experiment Station, Cornell University, Geneva, NY 14456, USA"
Journal Title:		Proc Natl Acad Sci U S A
Year:		2002
Volume:		99
Issue:		2
Page Number:		620 - 624
DOI:		10.1073/pnas.221601498
ISSN/ISBN:		0027-8424 (Print) 1091-6490 (Electronic) 0027-8424 (Linking)
Abstract:		"Moth species have evolved integral membrane desaturases that exhibit a wide diversity in substrate specificity, as well as in regiospecificity and stereospecificity of the unsaturated products. We report here the cloning and expression of a single desaturase from the sex pheromone gland of the light brown apple moth, Epiphyas postvittana, that makes E11 isomers of monounsaturated (E11-16 and E11-14) fatty acids and a diunsaturated (E9,E11-14) fatty acid. In the pheromone gland, the monoene precursor is made available by beta oxidation of E11-16 acid with a subsequent two-carbon loss to E9-14 acid. A functional assay using a baculovirus expression system required addition of myristic acid and E9-14 acid precursors to demonstrate the unusual regiospecificity and stereospecificity of this desaturase. The amino acid sequence of this desaturase has approximately 61% identity to that of Z11-desaturases from two other insect species, and only approximately 48% identity to the metabolic Z9-desaturases in those species. A pheromone-gland Z9-desaturase gene also was found with the light brown apple moth that differed in its deduced amino acid sequence (66% identity) with the metabolic Z9-desaturase from fat body in this species"
Keywords:		"Amino Acid Sequence Animals Baculoviridae/genetics Base Sequence Cell Line Cloning, Molecular DNA, Complementary/genetics Fat Body/enzymology Fatty Acid Desaturases/*genetics/*metabolism Fatty Acids, Unsaturated/biosynthesis/chemistry Female Gene Expressi;"
Notes:		"MedlineLiu, Weitian Jiao, Hongmei Murray, Nancy C O'Connor, Marion Roelofs, Wendell L eng Research Support, U.S. Gov't, Non-P.H.S. 2002/01/24 Proc Natl Acad Sci U S A. 2002 Jan 22; 99(2):620-4. doi: 10.1073/pnas.221601498"