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J Agric Food Chem

Title:		Glutathione S-Transferase Highly Expressed in Holotrichia parallela Antennae Inactivates the Odorant Unsaturated Aldehyde Volatiles
Author(s):		Liu JN; Xi JH; Wang Z; Zhao SW; Wang X; Bu YW; Zhou KX; Pan Y; Wang S;
Address:		"College of Plant Science, Jilin University, Changchun, Jilin 130062, People's Republic of China. Changchun Customs Technology Center, Changchun, Jilin 130062, People's Republic of China"
Journal Title:		J Agric Food Chem
Year:		2023
Volume:		20230531
Issue:		23
Page Number:		8797 - 8807
DOI:		10.1021/acs.jafc.3c00915
ISSN/ISBN:		1520-5118 (Electronic) 0021-8561 (Linking)
Abstract:		"Odorant-degrading enzymes in insects play a vital role in maintaining olfactory sensitivity. However, the role and molecular mechanism of glutathione S-transferases (GSTs) in odorant inactivation has been rarely studied. In the present study, 31 GSTs were identified from the antennal transcriptome of Holotrichia parallela. HpGSTd1 possesses the highest transcriptome expression level. Recombinant HpGSTd1 showed degradation activity toward various unsaturated aldehyde volatiles. Furthermore, the metabolite of cinnamaldehyde was identified by high-resolution mass spectrometry (HRMS). The molecular docking analysis and site-directed mutagenesis revealed the key residues of HpGSTd1 in degrading odorants. In addition, the unsaturated aldehyde volatiles elicited the behavioral and electrophysiological responses of H. parallela. Taken together, our findings suggest that HpGSTd1 may play an essential role in inactivating odorants in H. parallela, which provides new insights for identifying molecular targets and exploring effective olfactory regulators for this underground pest"
Keywords:		"Animals Odorants Glutathione Transferase/genetics/metabolism Aldehydes/metabolism Molecular Docking Simulation Arthropod Antennae/metabolism Insect Proteins/metabolism *Coleoptera/metabolism *Receptors, Odorant/genetics/metabolism Holotrichia parallela gl;"
Notes:		"MedlineLiu, Jia-Nan Xi, Jing-Hui Wang, Zhun Zhao, Shi-Wen Wang, Xiao Bu, Yun-Wei Zhou, Ke-Xin Pan, Yu Wang, Shang eng 2023/05/31 J Agric Food Chem. 2023 Jun 14; 71(23):8797-8807. doi: 10.1021/acs.jafc.3c00915. Epub 2023 May 31"