| Title: | Effect of pheromone induction on transfer of the Enterococcus faecalis plasmid pCF10 in intestinal mucus ex vivo |
| Author(s): | Licht TR; Hammerum AM; Jensen LB; Jacobsen BL; |
| Address: | "Danish Veterinary and Food Administration, Institute of Food Safety and Toxicology, Soborg, Denmark. trl@fdir.dk" |
| DOI: | 10.1111/j.1574-6968.2001.tb10902.x |
| ISSN/ISBN: | 0378-1097 (Print) 0378-1097 (Linking) |
| Abstract: | "The effect of synthetic sex pheromone on pheromone-inducible conjugation between the isogenic Enterococcus faecalis strains OG1RF and OG1SS was investigated in (i) Todd-Hewitt broth medium and (ii) intestinal mucus isolated from germ-free rats. In broth, the presence of synthetic pheromone cCF10 had no detectable effect on the transfer kinetics observed for the tetracycline resistance encoding plasmid pCF10. In mucus, presence of the same pheromone significantly increased the transfer efficiency observed during the first 2 h of conjugation, while the effect was less pronounced later in the experiment. We suggest that due to differences in diffusion rates and medium-binding of the pheromones, the effect of the synthetic cCF10 was immediately dominated by the effect of pheromones produced by the recipient E. faecalis strain in broth, while this happened later in mucus" |
| Keywords: | "Cecum/*microbiology *Conjugation, Genetic Culture Media Enterococcus faecalis/genetics/growth & development Mucus/*microbiology Oligopeptides/chemical synthesis/genetics/*metabolism Pheromones/chemical synthesis/genetics/*metabolism Plasmids/*genetics;" |
| Notes: | "MedlineLicht, T R Hammerum, A M Jensen, L B Jacobsen, B L eng Research Support, Non-U.S. Gov't England 2001/12/04 FEMS Microbiol Lett. 2001 Nov 13; 204(2):305-9. doi: 10.1111/j.1574-6968.2001.tb10902.x" |
