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Theriogenology

Title:		"Heat stress-induced autophagy promotes lactate secretion in cultured immature boar Sertoli cells by inhibiting apoptosis and driving SLC2A3, LDHA, and SLC16A1 expression"
Author(s):		Bao ZQ; Liao TT; Yang WR; Wang Y; Luo HY; Wang XZ;
Address:		"Chongqing Key Laboratory of Forage and Herbivore, College of Animal Science and Technology, Southwest University, Chongqing, P. R. China. Chongqing Key Laboratory of Forage and Herbivore, College of Animal Science and Technology, Southwest University, Chongqing, P. R. China; Research School of Engineering, College of Engineering and Computer Science, The Australian National University, Acton, ACT, Australia. College of Resource and Environment, Southwest University, Chongqing, P. R. China. Chongqing Key Laboratory of Forage and Herbivore, College of Animal Science and Technology, Southwest University, Chongqing, P. R. China. Electronic address: xianzhong_wang@aliyun.com"
Journal Title:		Theriogenology
Year:		2017
Volume:		20160915
Issue:
Page Number:		339 - 348
DOI:		10.1016/j.theriogenology.2016.09.016
ISSN/ISBN:		1879-3231 (Electronic) 0093-691X (Linking)
Abstract:		"This study aimed to determine whether heat stress (HS) could induce autophagy in immature boar Sertoli cells (SCs) and test whether HS-induced autophagy could regulate lactate secretion by SCs. Cultured immature boar SCs were incubated at 43 degrees C for 30 minutes. The ratio of LC3B-II to LC3B-I and the mRNA transcript levels of LC3B showed time-dependent changes 0 to 48 hours after HS treatment, which peaked at 24 hours and increased by 30.25% or 260%, respectively, compared with control SCs. The density of autolysosomes, which were labeled with a red dye, was higher at 24 hours than at any other time point. However, the apoptosis rate, cleavage of caspase-3, and mRNA transcript levels of CASP3 (caspase-3) at 24 hours after HS were lower than at 12 hours. Furthermore, lactate secretion, and mRNA transcript levels of SLC2A3 (GLUT3), LDHA (LDHA), and SLC16A1 (MCT1) also showed time-dependent changes with a peak at 24 hours. In addition, LY294002 (20 muM) significantly inhibited changes in ratio of LC3B-II to LC3B-I, LC3B mRNA transcript levels, and autolysosome formation. It also resulted in significantly less lactate secretion and increased apoptosis but showed no effect on B-cell lymphoma-2 expression in heat-treated immature SCs. These findings indicated that HS-induced autophagy regulates lactate secretion by inhibiting apoptosis and increasing mRNA transcript and protein levels of SLC2A3, LDHA, and SLC16A1, which suggests that HS-induced autophagy may enhance lactate secretion by SCs"
Keywords:		Animals Apoptosis/physiology Autophagy/physiology Gene Expression Regulation Glucose Transporter Type 3/genetics/*metabolism Hot Temperature/*adverse effects Isoenzymes/genetics/metabolism L-Lactate Dehydrogenase/genetics/*metabolism Lactate Dehydrogenase;
Notes:		"MedlineBao, Zi-Qiang Liao, Ting-Ting Yang, Wei-Rong Wang, Yi Luo, Hong-Yan Wang, Xian-Zhong eng 2016/10/19 Theriogenology. 2017 Jan 1; 87:339-348. doi: 10.1016/j.theriogenology.2016.09.016. Epub 2016 Sep 15"