| Title: | Exhaled Volatile Organic Compounds for Identifying Patients With Chronic Pulmonary Aspergillosis |
| Author(s): | Li ZT; Zeng PY; Chen ZM; Guan WJ; Wang T; Lin Y; Li SQ; Zhang ZJ; Zhan YQ; Wang MD; Tan GB; Li X; Ye F; |
| Address: | "State Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, National Center for Respiratory Medicine, Guangzhou Institute of Respiratory Health, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, China. Department of Thoracic Surgery, Guangzhou Institute for Respiratory Health, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, Guangdong, China. Institute of Mass Spectrometry and Atmospheric Environment, Jinan University, Guangzhou, China. Guangdong Provincial Engineering Research Center for On-Line Source Apportionment System of Air Pollution, Guangzhou, China. College of Pharmacy, Hena University of Chinese Medicine, Zhengzhou, China. Guangzhou Hexin Instrument Co., Ltd., Guangzhou, China" |
| ISSN/ISBN: | 2296-858X (Print) 2296-858X (Electronic) 2296-858X (Linking) |
| Abstract: | "Background: Diagnosing chronic pulmonary aspergillosis is a major challenge in clinical practice. The development and validation of a novel, sensitive and specific assay for diagnosing chronic pulmonary aspergillosis is urgently needed. Methods: From April 2018 to June 2019, 53 patients with chronic pulmonary aspergillosis (CPA), 32 patients with community-acquired pneumonia (CAP) and 48 healthy controls were recruited from the First Affiliated Hospital of Guangzhou Medical University. Clinical characteristics and samples were collected at enrollment. All exhaled breath samples were analyzed offline using thermal desorption single-photon ionization time-of-flight mass spectrometry; to analyze the metabolic pathways of the characteristic volatile organic compounds, serum samples were subjected to ultrahigh-performance liquid chromatography. Results: We identified characteristic volatile organic compounds in patients with chronic pulmonary aspergillosis, which mainly consisted of phenol, neopentyl alcohol, toluene, limonene and ethylbenzene. These compounds were assessed using a logistic regression model. The sensitivity and specificity were 95.8 and 96.9% for discriminating patients in the CPA group from those in the CAP group and 95.8 and 97.9% for discriminating patients in the CPA group from healthy controls, respectively. The concentration of limonene (m/z 136) correlated significantly positively with anti-Aspergillus fumigatus IgG antibody titers (r = 0.420, P < 0.01). After antifungal treatment, serum IgG and the concentration of limonene (m/z 136) decreased in the subgroup of patients with chronic pulmonary aspergillosis. Conclusions: We identified VOCs that can be used as biomarkers for differential diagnosis and therapeutic response prediction in patients with chronic pulmonary aspergillosis" |
| Keywords: | chronic pulmonary aspergillosis exhalation metabolomics single-photon ionization-mass spectrometry volatile organic compounds; |
| Notes: | "PubMed-not-MEDLINELi, Zheng-Tu Zeng, Pei-Ying Chen, Zhao-Ming Guan, Wei-Jie Wang, Tong Lin, Ye Li, Shao-Qiang Zhang, Zhi-Juan Zhan, Yang-Qing Wang, Ming-Die Tan, Guo-Bin Li, Xue Ye, Feng eng Switzerland 2021/10/12 Front Med (Lausanne). 2021 Sep 23; 8:720119. doi: 10.3389/fmed.2021.720119. eCollection 2021" |
