| Title: | "cDNA cloning and sequence determination of the pheromone biosynthesis activating neuropeptide from the seabuckthorn carpenterworm, Holcocerus hippophaecolus (Lepidoptera: Cossidae)" |
| Author(s): | Li J; Zhou J; Sun R; Zhang H; Zong S; Luo Y; Sheng X; Weng Q; |
| Address: | "Laboratory of Animal Physiology, College of Biological Science and Technology, Beijing Forestry University, Beijing, People's Republic of China" |
| Journal Title: | Arch Insect Biochem Physiol |
| ISSN/ISBN: | 1520-6327 (Electronic) 0739-4462 (Linking) |
| Abstract: | "The PBAN (pheromone biosynthesis activating neuropeptide)/pyrokinin peptides comprise a major neuropeptide family characterized by a common FXPRL amide at the C-terminus. These peptides are actively involved in many essential endocrine functions. For the first time, we reported the cDNA cloning and sequence determination of the PBAN from the seabuckthorn carpenterworm, Holcocerus hippophaecolus, by using rapid amplification of cDNA ends. The full-length cDNA of Hh-DH-PBAN contained five peptides: diapause hormone (DH) homolog, alpha-neuropeptide (NP), beta-NP, PBAN, and gamma-NP. All of the peptides were amidated at their C-terminus and shared a conserved motif, FXPR (or K) L. Moreover, Hh-DH-PBAN had high homology to the other members of the PBAN peptide family: 56% with Manduca sexta, 66% with Bombyx mori, 77% with Helicoverpa zea, and 47% with Plutella xylostella. Phylogenetic analysis revealed that Hh-DH-PBAN was closely related to PBANs from Noctuidae, demonstrated by the relatively higher similarity compared with H. zea. In addition, real-time quantitative PCR (qRT-PCR) analysis showed that Hh-DH-PBAN mRNA expression peaked in the brain-subesophageal ganglion (Br-SOG) complex, and was also detected at high levels during larval and adult stages. The expression decreased significantly after pupation. These results provided information concerning molecular structure characteristics of Hh-DH-PBAN, whose expression profile suggested that the Hh-DH-PBAN gene might be correlated with larval development and sex pheromone biosynthesis in females of the H. hippophaecolus" |
| Keywords: | "Amino Acid Sequence Animals Base Sequence Cloning, Molecular Female Gene Expression *Hippophae Larva/metabolism Molecular Sequence Data Moths/*physiology Neuropeptides/*biosynthesis/genetics Phylogeny RNA, Messenger/genetics Sequence Analysis, DNA Sequenc;" |
| Notes: | "MedlineLi, Juan Zhou, Jiao Sun, Rongbo Zhang, Haolin Zong, Shixiang Luo, Youqing Sheng, Xia Weng, Qiang eng Research Support, Non-U.S. Gov't 2013/01/31 Arch Insect Biochem Physiol. 2013 Apr; 82(4):183-95. doi: 10.1002/arch.21084. Epub 2013 Jan 29" |
